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A serial dilution microfluidic device using a ladder network generating logarithmic or linear concentrations

Authors
Kim, ChoongLee, KangsunKim, Jong HyunShin, Kyeong SikLee, Kyu-JungKim, Tae SongKang, Ji Yoon
Issue Date
2008
Publisher
ROYAL SOC CHEMISTRY
Citation
LAB ON A CHIP, v.8, no.3, pp 473 - 479
Pages
7
Indexed
SCIE
SCOPUS
Journal Title
LAB ON A CHIP
Volume
8
Number
3
Start Page
473
End Page
479
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/125531
DOI
10.1039/b714536e
ISSN
1473-0197
1473-0189
Abstract
In this paper, we propose a serial dilution microfluidic chip which is able to generate logarithmic or linear step-wise concentrations. These concentrations were generated via adjustments in the flow rate of two converging fluids at the channel junctions of the ladder network. The desired dilution ratios are almost independent of the flow rate or diffusion length of molecules, as the dilution device is influenced only by the ratio of volumetric flow rates. Given a set of necessary dilution ratios, whether linear or logarithmic, a serial dilution chip can be constructed via the modification of a microfluidic resistance network. The design principle was suggested and both the logarithmic and linear dilution chips were fabricated in order to verify their performance in accordance with the fluorescence intensity. The diluted concentrations of a fluorescein solution in the microfluidic device evidenced relatively high linearity, and the cytotoxicity test of MCF-7 breast cancer cells via the logarithmic dilution chip was generally consistent with the results generated with manual dilution.
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