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Modulation of biodistribution and expression of plasmid DNA following mesenchymal progenitor cell-based delivery

Authors
Jeong, Young-SinKim, Eun JoongShim, Chang-KooHou, Joon HyukKim, Jung MoggChoi, Han-GonKim, Won-KiOh, Yu-Kyoung
Issue Date
2008
Publisher
TAYLOR & FRANCIS LTD
Keywords
mesenchymal progenitor cells; plasmid DNA; gene delivery vehicles; biodistribution; expression
Citation
JOURNAL OF DRUG TARGETING, v.16, no.5, pp.405 - 414
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF DRUG TARGETING
Volume
16
Number
5
Start Page
405
End Page
414
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/125609
DOI
10.1080/10611860802088713
ISSN
1061-186X
Abstract
Although therapeutic applications of mesenchymal progenitor cells (MPCs) have been studied, the in vivo fate of genes delivered by the MPCs has received little attention. We report here the in vivo kinetics, tissue distribution, and duration of gene expression after systemic administration of plasmid DNA delivered by MPCs. Murine MPCs were isolated from bone marrow, cultured, and transfected with plasmid DNA using polyethylenimine. The gene-modified MPCs or naked plasmid DNA was administered intravenously to mice. Injected MPCs incorporating plasmid DNA yielded elevated serum concentrations when compared with the group treated with plasmid DNA alone, a 280-fold higher level measured at 5-min post-administration. Moreover, plasmid DNA delivered in MPCs was detected in several organs, lymph nodes, and bone marrow. The highest levels of distribution were observed in the liver, followed by lung and spleen at 4 days post-dose. Similar to the distribution of DNA, significant expression levels of the exogenous gene were observed only after delivery of the DNA in MPCs, demonstrating the sustained expression at the liver, lung, and kidney for 4 days after tail vein injection. This study provides perspectives regarding the in vivo fate and target tissue distribution of genes following MPC-based delivery.
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Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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