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Gene expression profiling in lung tissues from rats exposed to formaldehyde

Authors
Sul, DonggeunKim, HyunsookOh, EunhaPhark, SoheeCho, EunkyungChoi, SeonyoungKang, Hyung-SikKim, Eun-MiHwang, Kwang-WooJung, Woon-Won
Issue Date
8월-2007
Publisher
SPRINGER HEIDELBERG
Keywords
DNA damage; formaldehyde; genomics; lipid peroxidation; protein oxidation; rat lung
Citation
ARCHIVES OF TOXICOLOGY, v.81, no.8, pp.589 - 597
Indexed
SCIE
SCOPUS
Journal Title
ARCHIVES OF TOXICOLOGY
Volume
81
Number
8
Start Page
589
End Page
597
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/125736
DOI
10.1007/s00204-007-0182-9
ISSN
0340-5761
Abstract
Formaldehyde is a ubiquitous toxic organic compound recently classified as a carcinogen by the International Agency for Research on Cancer and one of the major factors causing sick building syndrome. In this study, we have investigated the effects of formaldehyde on mRNA expression in rat lung tissues by applying genomics. Rats were exposed to ambient air and two different concentrations of formaldehyde (0, 5, 10 ppm) for 2 weeks at 6 h/day and 5 days/week in an inhalation chamber. Malondialdehyde (MDA) assay and carbonyl spectrometric assay were conducted to determine lipid peroxidation and protein oxidation levels and Comet assays were used for genotoxicity evaluation. Level of MDA, carbonyl insertion and DNA damage in the lungs of rats exposed to FA were found to be dose dependently increased. Gene expression was evaluated by using a bio-array hybridization analysis. A total of 21 (2 up- and 19 down-regulated) genes were identified as biomarkers for formaldehyde effects. Several differentiated gene groups were found. Genes involved in apoptosis, immunity, metabolism, signal transduction, transportation, coagulation and oncogenesis were found to be up- and down-regulated. Among these genes, the mRNA expressions of cytochrome P450, hydroxymethylbilane synthase, glutathione reductase, carbonic anhydrase 2, natriuretic peptide receptor 3, lysosomal associated protein transmembrane 5, regulator of G-protein signaling 3, olfactomedin related ER localized protein, and poly (ADP-ribose) polymerase-1 were confirmed by quantitative RT-PCR analysis. In summary, the MDA lipid peroxidation and the carbonyl protein oxidation assays showed that cytotoxic effects increased with increasing formaldehyde levels. Genomic analysis showed that 21 genes were up- or down-regulated. Of these genes, nine were confirmed by quantitative RT-PCR and could be potential biomarkers for human diseases associated with formaldehyde exposure.
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