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Biological imaging of HEK293 cells expressing PLC gamma 1 using surface-enhanced raman microscopy

Authors
Lee, SangyeopKim, SungyongChoo, JaebumShin, Soon YoungLee, Young HanChoi, Ha YoungHa, SeunghanKang, KyunghoOh, Chil Hwan
Issue Date
1-2월-2007
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v.79, no.3, pp.916 - 922
Indexed
SCIE
SCOPUS
Journal Title
ANALYTICAL CHEMISTRY
Volume
79
Number
3
Start Page
916
End Page
922
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/125817
DOI
10.1021/ac061246a
ISSN
0003-2700
Abstract
Surface-enhanced Raman scattering (SERS) imaging has been used for the targeting and imaging of specific cancer markers in live cells. For this purpose, Au/Ag core-shell nanoparticles, conjugated with monoclonal antibodies, were prepared. The procedures to label live cells with those bimetallic nanoprobes have been developed and used for highly sensitive SERS imaging of live cells. In the present study, live HEK293 cells expressing PLC gamma 1 have been used as the optical imaging target. Our results demonstrate the potential feasibility of SERS imaging technology for the highly sensitive imaging of cancer biomarkers in live cells.
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