Stimulating effect of whey protein hydrolysate on bone growth in MC3T3-E1 cells and a rat model
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, Joo Hyun | - |
dc.contributor.author | Kim, Singeun | - |
dc.contributor.author | Lee, Hyun Jung | - |
dc.contributor.author | Suh, Hyung Joo | - |
dc.contributor.author | Jo, Kyungae | - |
dc.date.accessioned | 2021-11-18T09:40:45Z | - |
dc.date.available | 2021-11-18T09:40:45Z | - |
dc.date.created | 2021-08-30 | - |
dc.date.issued | 2021-06-07 | - |
dc.identifier.issn | 2042-650X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/127859 | - |
dc.description.abstract | This study was conducted to investigate the effect of whey protein hydrolysate (WPH) on osteogenic cell differentiation and its growth-promoting effects in rats. Alkaline phosphatase (ALP) activity and calcium deposition were measured by treating MC3T3-E1 cells with WPH, and mRNA and protein levels of factors related to osteoblast differentiation were assessed. ALP activity and calcium deposition were significantly increased in the WPH group (p < 0.001). These findings were confirmed by the upregulation of ALP, bone morphogenic protein, bone sialoprotein, and collagen at the mRNA and protein levels. Furthermore, to confirm the growth-promoting effect of WPH, bone growth was analyzed by administering 3-week-old Sprague-Dawley rats with whey protein or WPH. Moreover, serum levels of calcium, ALP, and insulin-like growth factor-1 (IGF-1) were analyzed, bone analysis was performed using micro-CT, and the size of the growth plate was measured by Cresyl violet staining. When rats were administered with a high dose of WPH (600 mg per kg per day), calcium levels decreased significantly, while ALP levels (1.14-fold; p < 0.01), IGF-1 levels, tibia length, and growth plate height increased significantly compared to those in the control group. Collectively, WPH has shown to be effective in bone differentiation and bone growth. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | OSTEOBLAST DIFFERENTIATION | - |
dc.subject | ALKALINE-PHOSPHATASE | - |
dc.subject | BIOACTIVE PEPTIDES | - |
dc.subject | IN-VITRO | - |
dc.subject | MINERALIZATION | - |
dc.subject | PROLIFERATION | - |
dc.subject | EXPRESSION | - |
dc.subject | MILK | - |
dc.subject | INVITRO | - |
dc.title | Stimulating effect of whey protein hydrolysate on bone growth in MC3T3-E1 cells and a rat model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Suh, Hyung Joo | - |
dc.identifier.doi | 10.1039/d1fo00546d | - |
dc.identifier.scopusid | 2-s2.0-85107828059 | - |
dc.identifier.wosid | 000648615700001 | - |
dc.identifier.bibliographicCitation | FOOD & FUNCTION, v.12, no.11, pp.5109 - 5117 | - |
dc.relation.isPartOf | FOOD & FUNCTION | - |
dc.citation.title | FOOD & FUNCTION | - |
dc.citation.volume | 12 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 5109 | - |
dc.citation.endPage | 5117 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | OSTEOBLAST DIFFERENTIATION | - |
dc.subject.keywordPlus | ALKALINE-PHOSPHATASE | - |
dc.subject.keywordPlus | BIOACTIVE PEPTIDES | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | MINERALIZATION | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MILK | - |
dc.subject.keywordPlus | INVITRO | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.