Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Soo-Ji | - |
dc.contributor.author | Jeong, Tae Yeong | - |
dc.contributor.author | Shin, Seung Kyun | - |
dc.contributor.author | Yoon, Da Eun | - |
dc.contributor.author | Lim, Soo-Yeon | - |
dc.contributor.author | Kim, Sol Pin | - |
dc.contributor.author | Choi, Jungmin | - |
dc.contributor.author | Lee, Hyunji | - |
dc.contributor.author | Hong, Jeong-Im | - |
dc.contributor.author | Ahn, Jinhee | - |
dc.contributor.author | Seong, Je Kyung | - |
dc.contributor.author | Kim, Kyoungmi | - |
dc.date.accessioned | 2021-11-18T10:41:07Z | - |
dc.date.available | 2021-11-18T10:41:07Z | - |
dc.date.created | 2021-08-30 | - |
dc.date.issued | 2021-06-03 | - |
dc.identifier.issn | 1474-760X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/127866 | - |
dc.description.abstract | Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing. Herein, we introduce two strategies to improve the editing efficiency using proximal dead sgRNA and chromatin-modulating peptides. We used enhanced prime-editing to generate Igf2 mutant mice with editing frequencies of up to 47% and observed germline transmission, no off-target effects, and a dwarf phenotype. This improved prime-editing method can be efficiently applied to cell research and to generate mouse models. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.subject | GENOMIC DNA | - |
dc.subject | BASE | - |
dc.subject | GENERATION | - |
dc.subject | ACTIVATION | - |
dc.title | Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Jungmin | - |
dc.identifier.doi | 10.1186/s13059-021-02389-w | - |
dc.identifier.scopusid | 2-s2.0-85107198784 | - |
dc.identifier.wosid | 000660859100002 | - |
dc.identifier.bibliographicCitation | GENOME BIOLOGY, v.22, no.1 | - |
dc.relation.isPartOf | GENOME BIOLOGY | - |
dc.citation.title | GENOME BIOLOGY | - |
dc.citation.volume | 22 | - |
dc.citation.number | 1 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.subject.keywordPlus | GENOMIC DNA | - |
dc.subject.keywordPlus | BASE | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordAuthor | Prime editor | - |
dc.subject.keywordAuthor | Igf2 | - |
dc.subject.keywordAuthor | Adamts20 | - |
dc.subject.keywordAuthor | Mouse cells and embryos | - |
dc.subject.keywordAuthor | Germline transmission | - |
dc.subject.keywordAuthor | Dwarf phenotype | - |
dc.subject.keywordAuthor | Proximal dead sgRNA | - |
dc.subject.keywordAuthor | Chromatin-modulating peptides | - |
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