Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor
- Authors
- Park, Soo-Ji; Jeong, Tae Yeong; Shin, Seung Kyun; Yoon, Da Eun; Lim, Soo-Yeon; Kim, Sol Pin; Choi, Jungmin; Lee, Hyunji; Hong, Jeong-Im; Ahn, Jinhee; Seong, Je Kyung; Kim, Kyoungmi
- Issue Date
- 3-6월-2021
- Publisher
- BMC
- Keywords
- Prime editor; Igf2; Adamts20; Mouse cells and embryos; Germline transmission; Dwarf phenotype; Proximal dead sgRNA; Chromatin-modulating peptides
- Citation
- GENOME BIOLOGY, v.22, no.1
- Indexed
- SCIE
SCOPUS
- Journal Title
- GENOME BIOLOGY
- Volume
- 22
- Number
- 1
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/127866
- DOI
- 10.1186/s13059-021-02389-w
- ISSN
- 1474-760X
- Abstract
- Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing. Herein, we introduce two strategies to improve the editing efficiency using proximal dead sgRNA and chromatin-modulating peptides. We used enhanced prime-editing to generate Igf2 mutant mice with editing frequencies of up to 47% and observed germline transmission, no off-target effects, and a dwarf phenotype. This improved prime-editing method can be efficiently applied to cell research and to generate mouse models.
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- Appears in
Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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