Non-invasive in vivo monitoring of transplanted stem cells in 3D-bioprinted constructs using near-infrared fluorescent imaging
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Soon Hee | - |
dc.contributor.author | Kwon, Jin Seon | - |
dc.contributor.author | Cho, Jae Gu | - |
dc.contributor.author | Park, Kate G. | - |
dc.contributor.author | Lim, Tae Hyeon | - |
dc.contributor.author | Kim, Moon Suk | - |
dc.contributor.author | Choi, Hak Soo | - |
dc.contributor.author | Park, Chan Hum | - |
dc.contributor.author | Lee, Sang Jin | - |
dc.date.accessioned | 2021-11-20T17:40:54Z | - |
dc.date.available | 2021-11-20T17:40:54Z | - |
dc.date.created | 2021-08-30 | - |
dc.date.issued | 2021-05 | - |
dc.identifier.issn | 2380-6761 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/128141 | - |
dc.description.abstract | Cell-based tissue engineering strategies have been widely established. However, the contributions of the transplanted cells within the tissue-engineered scaffolds to the process of tissue regeneration remain poorly understood. Near-infrared (NIR) fluorescence imaging systems have great potential to non-invasively monitor the transplanted cell-based tissue constructs. In this study, labeling mesenchymal stem cells (MSCs) using a lipophilic pentamethine indocyanine (CTNF127, emission at 700 nm) as a NIR fluorophore was optimized, and the CTNF127-labeled MSCs (NIR-MSCs) were printed embedding in gelatin methacryloyl bioink. The NIR-MSCs-loaded bioink showed excellent printability. In addition, NIR-MSCs in the 3D constructs showed high cell viability and signal stability for an extended period in vitro. Finally, we were able to non-invasively monitor the NIR-MSCs in constructs after implantation in a rat calvarial bone defect model, and the transplanted cells contributed to tissue formation without specific staining. This NIR-based imaging system for non-invasive cell monitoring in vivo could play an active role in validating the cell fate in cell-based tissue engineering applications. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | Non-invasive in vivo monitoring of transplanted stem cells in 3D-bioprinted constructs using near-infrared fluorescent imaging | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Cho, Jae Gu | - |
dc.identifier.doi | 10.1002/btm2.10216 | - |
dc.identifier.scopusid | 2-s2.0-85103170837 | - |
dc.identifier.wosid | 000632929800001 | - |
dc.identifier.bibliographicCitation | BIOENGINEERING & TRANSLATIONAL MEDICINE, v.6, no.2 | - |
dc.relation.isPartOf | BIOENGINEERING & TRANSLATIONAL MEDICINE | - |
dc.citation.title | BIOENGINEERING & TRANSLATIONAL MEDICINE | - |
dc.citation.volume | 6 | - |
dc.citation.number | 2 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | AMNIOTIC-FLUID | - |
dc.subject.keywordPlus | BONE-FORMATION | - |
dc.subject.keywordPlus | DEFECTS | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.subject.keywordPlus | SCAFFOLD | - |
dc.subject.keywordPlus | STROMAL CELLS | - |
dc.subject.keywordPlus | THICKNESS | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordAuthor | infrared fluorescence | - |
dc.subject.keywordAuthor | invasive monitoring | - |
dc.subject.keywordAuthor | near& | - |
dc.subject.keywordAuthor | #8208 | - |
dc.subject.keywordAuthor | non& | - |
dc.subject.keywordAuthor | #8208 | - |
dc.subject.keywordAuthor | scaffold monitoring | - |
dc.subject.keywordAuthor | stem cell tracking | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.