Non-invasive in vivo monitoring of transplanted stem cells in 3D-bioprinted constructs using near-infrared fluorescent imaging
- Authors
- Kim, Soon Hee; Kwon, Jin Seon; Cho, Jae Gu; Park, Kate G.; Lim, Tae Hyeon; Kim, Moon Suk; Choi, Hak Soo; Park, Chan Hum; Lee, Sang Jin
- Issue Date
- 5월-2021
- Publisher
- WILEY
- Keywords
- infrared fluorescence; invasive monitoring; near& #8208; non& #8208; scaffold monitoring; stem cell tracking
- Citation
- BIOENGINEERING & TRANSLATIONAL MEDICINE, v.6, no.2
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOENGINEERING & TRANSLATIONAL MEDICINE
- Volume
- 6
- Number
- 2
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/128141
- DOI
- 10.1002/btm2.10216
- ISSN
- 2380-6761
- Abstract
- Cell-based tissue engineering strategies have been widely established. However, the contributions of the transplanted cells within the tissue-engineered scaffolds to the process of tissue regeneration remain poorly understood. Near-infrared (NIR) fluorescence imaging systems have great potential to non-invasively monitor the transplanted cell-based tissue constructs. In this study, labeling mesenchymal stem cells (MSCs) using a lipophilic pentamethine indocyanine (CTNF127, emission at 700 nm) as a NIR fluorophore was optimized, and the CTNF127-labeled MSCs (NIR-MSCs) were printed embedding in gelatin methacryloyl bioink. The NIR-MSCs-loaded bioink showed excellent printability. In addition, NIR-MSCs in the 3D constructs showed high cell viability and signal stability for an extended period in vitro. Finally, we were able to non-invasively monitor the NIR-MSCs in constructs after implantation in a rat calvarial bone defect model, and the transplanted cells contributed to tissue formation without specific staining. This NIR-based imaging system for non-invasive cell monitoring in vivo could play an active role in validating the cell fate in cell-based tissue engineering applications.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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