Fermented Gamma Aminobutyric Acid Improves Sleep Behaviors in Fruit Flies and Rodent Models
- Authors
- Jeong, A-Hyun; Hwang, Jisu; Jo, Kyungae; Kim, Singeun; Ahn, Yejin; Suh, Hyung Joo; Choi, Hyeon-Son
- Issue Date
- 4월-2021
- Publisher
- MDPI
- Keywords
- & #947; -aminobutyric acid; GABA receptor; sleep promotion; non-rapid eye movement; Drosophila melanogaster; rodents
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.22, no.7
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Volume
- 22
- Number
- 7
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/128299
- DOI
- 10.3390/ijms22073537
- ISSN
- 1661-6596
- Abstract
- The aim of this study was to investigate the effect of Lactobacillus brevis-fermented gamma-aminobutyric acid (LB-GABA) on sleep behaviors in invertebrate and vertebrate models. In Drosophila melanogaster, LB-GABA-treated group showed an 8-9%-longer sleep duration than normal group did. LB-GABA-treated group also showed a 46.7% lower level of nighttime activity with a longer (11%) sleep duration under caffeine-induced arousal conditions. The LB-GABA-mediated inhibition of activity was confirmed as a reduction of total movement of flies using a video tracking system. In the pentobarbital-induced sleep test in mice, LB-GABA (100 mg/kg) shortened the time of onset of sleep by 32.2% and extended sleeping time by 59%. In addition, mRNA and protein level of GABAergic/Serotonergic neurotransmitters were upregulated following treatment with LB-GABA (2.0%). In particular, intestine- and brain-derived GABA(A) protein levels were increased by sevenfold and fivefold, respectively. The electroencephalography (EEG) analysis in rats showed that LB-GABA significantly increased non-rapid eye movement (NREM) (53%) with the increase in theta (theta, 59%) and delta (delta, 63%) waves, leading to longer sleep time (35%), under caffeine-induced insomnia conditions. LB-GABA showed a dose-dependent agonist activity on human GABA(A) receptor with a half-maximal effective concentration (EC50) of 3.44 mu g/mL in human embryonic kidney 293 (HEK293) cells.
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Collections - College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles
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