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A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of Orientia tsutsugamushi Using Multiplex PCR-Based Next-Generation Sequencing
- Authors
- Cho, Seungchan; Allison, Jon C.; Park, Kkothanahreum; No, Jin Sun; Lee, Seung-Ho; Park, Kyungmin; Kim, Jongwoo; Klein, Terry A.; Kim, Heung-Chul; Kim, Won-Keun; Song, Jin-Won
- Issue Date
- Apr-2021
- Publisher
- MDPI
- Keywords
- Orientia tsutsugamushi; scrub typhus; genetic identification; target enrichment next-generation sequencing
- Citation
- PATHOGENS, v.10, no.4
- Indexed
- SCIE
SCOPUS
- Journal Title
- PATHOGENS
- Volume
- 10
- Number
- 4
- ISSN
- 2076-0817
- Abstract
- An epidemiological investigation was conducted for a scrub typhus case reported in a U.S. Forces Korea military patient in the Republic of Korea in November 2018. The patient had a fever, maculopapular rash, and an eschar. The full-length sequence of Orientia tsutsugamushi 56-kDa type-specific antigen (TSA) gene was obtained from eschar tissue by multiplex PCR-based Next Generation Sequencing for genetic identification. Based on the 56-kDa TSA gene, the O. tsutsugamushi aligned most closely with the Boryong strain.
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Related Researcher
- Song, Jin Won
- Department of Biomedical Sciences