Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Extracellular Vesicles From a Three-Dimensional Culture of Perivascular Cells Accelerate Skin Wound Healing in a Rat

Authors
Kim, Min HoChung, ChanghoOh, Mun HoJun, Jin HyunKo, YongLee, Jong Hun
Issue Date
Oct-2021
Publisher
SPRINGER
Keywords
Perivascular cells; 3-dimensional culture; Conditioned medium; Extracellular vesicle; Wound healing
Citation
AESTHETIC PLASTIC SURGERY, v.45, no.5, pp 2437 - 2446
Pages
10
Indexed
SCIE
SCOPUS
Journal Title
AESTHETIC PLASTIC SURGERY
Volume
45
Number
5
Start Page
2437
End Page
2446
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/128600
DOI
10.1007/s00266-021-02254-y
ISSN
0364-216X
1432-5241
Abstract
Background Soluble proteins and extracellular vesicles (EVs) are crucial wound repair mediators in cell-based therapy. Previous studies reported that EVs of perivascular cells stimulated migration and proliferation of cell types involved in the dermatological wound healing process. However, these studies only show effects of EVs from perivascular cells (PVCs) for in vitro models. Methods EVs were collected from 3D-cultured PVC (PVC-3D-EV) and compared with EVs from 2D-culture PVC (PVC-2D-EV) to investigate effects on wound contraction, angiogenesis, activation of myofibroblast, and collagen deposition. Results PVC-3D-EV was significantly improved in terms of wound contraction compared with PVC-2D-EV and the control. Activation of myofibroblast and collagen deposition in a rat skin wound model was significantly stimulated by PVC-3D-EV. In addition, angiogenesis and vascular endothelial growth factor expression were also highly stimulated by PVC-3D-EV. These results suggest that PVC-3D-EV was regulated in granulation tissue formation, angiogenesis, and wound contraction in healing of a rat skin wound. These results indicate a pivotal role of PVC-3D-EV in wound healing through multiple mechanisms. Conclusions 3D-culture using a polystyrene scaffold is demonstrated to be a better system for providing better physiological conditions than the 2D-culture system, and EVs from 3D-cultured PVC could be a promising option for healing skin wound.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Life Sciences and Biotechnology > ETC > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE