Efficient Human Cell Coexpression System and Its Application to the Production of Multiple Coronavirus Antigens
- Authors
- Kim, C.; Jeong, Y.K.; Yu, J.; Shin, H.J.; Ku, K.B.; Cha, H.J.; Han, J.H.; Hong, S.-A.; Kim, B.-T.; Kim, S.-J.; Woo, J.-S.; Bae, S.
- Issue Date
- 4월-2021
- Publisher
- John Wiley and Sons Inc
- Keywords
- coronaviruses; CRISPR-Cas9; protein expression; targeted knock-in; vaccines
- Citation
- Advanced Biology, v.5, no.4
- Indexed
- SCIE
SCOPUS
- Journal Title
- Advanced Biology
- Volume
- 5
- Number
- 4
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/129021
- DOI
- 10.1002/adbi.202000154
- ISSN
- 2701-0198
- Abstract
- Coproduction of multiple proteins at high levels in a single human cell line would be extremely useful for basic research and medical applications. Here, a novel strategy for the stable expression of multiple proteins by integrating the genes into defined transcriptional hotspots in the human genome is presented. As a proof-of-concept, it is shown that EYFP is expressed at similar levels from hotspots and that the EYFP expression increases proportionally with the copy number. It is confirmed that three different fluorescent proteins, encoded by genes integrated at different loci, can be coexpressed at high levels. Further, a stable cell line is generated, producing antigens from different human coronaviruses: MERS-CoV and HCoV-OC43. Antibodies raised against these antigens, which contain human N-glycosylation, show neutralizing activities against both viruses, suggesting that the coexpression system provides a quick and predictable way to produce multiple coronavirus antigens, such as the recent 2019 novel human coronavirus. © 2021 The Authors. Advanced Biology published by Wiley-VCH GmbH
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