A therapeutic neutralizing antibody targeting receptor binding domain of SARS-CoV-2 spike protein
- Authors
- Kim, C.; Ryu, D.-K.; Lee, J.; Kim, Y.-I.; Seo, J.-M.; Kim, Y.-G.; Jeong, J.-H.; Kim, M.; Kim, J.-I.; Kim, P.; Bae, J.S.; Shim, E.Y.; Lee, M.S.; Kim, M.S.; Noh, H.; Park, G.-S.; Park, J.S.; Son, D.; An, Y.; Lee, J.N.; Kwon, K.-S.; Lee, J.-Y.; Lee, H.; Yang, J.-S.; Kim, K.-C.; Kim, S.S.; Woo, H.-M.; Kim, J.-W.; Park, M.-S.; Yu, K.-M.; Kim, S.-M.; Kim, E.-H.; Park, S.-J.; Jeong, S.T.; Yu, C.H.; Song, Y.; Gu, S.H.; Oh, H.; Koo, B.-S.; Hong, J.J.; Ryu, C.-M.; Park, W.B.; Oh, M.-D.; Choi, Y.K.; Lee, S.-Y.
- Issue Date
- 12-1월-2021
- Publisher
- Nature Research
- Citation
- Nature Communications, v.12, no.1
- Indexed
- SCIE
SCOPUS
- Journal Title
- Nature Communications
- Volume
- 12
- Number
- 1
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/129386
- DOI
- 10.1038/s41467-020-20602-5
- ISSN
- 2041-1723
- Abstract
- Vaccines and therapeutics are urgently needed for the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we screen human monoclonal antibodies (mAb) targeting the receptor binding domain (RBD) of the viral spike protein via antibody library constructed from peripheral blood mononuclear cells of a convalescent patient. The CT-P59 mAb potently neutralizes SARS-CoV-2 isolates including the D614G variant without antibody-dependent enhancement effect. Complex crystal structure of CT-P59 Fab/RBD shows that CT-P59 blocks interaction regions of RBD for angiotensin converting enzyme 2 (ACE2) receptor with an orientation that is notably different from previously reported RBD-targeting mAbs. Furthermore, therapeutic effects of CT-P59 are evaluated in three animal models (ferret, hamster, and rhesus monkey), demonstrating a substantial reduction in viral titer along with alleviation of clinical symptoms. Therefore, CT-P59 may be a promising therapeutic candidate for COVID-19. © 2021, The Author(s).
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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