Histamine Induced Production of Chemokine CXCL8 Through H1R/PLC and NF-κB Signaling Pathways in Nasal Fibroblasts
DC Field | Value | Language |
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dc.contributor.author | 강병진 | - |
dc.contributor.author | 박주후 | - |
dc.contributor.author | 이흥만 | - |
dc.date.accessioned | 2021-12-12T18:41:18Z | - |
dc.date.available | 2021-12-12T18:41:18Z | - |
dc.date.created | 2021-08-31 | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 1229-1498 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/131115 | - |
dc.description.abstract | Background and Objectives: Histamine has been suggested to play an important role during allergic and inflammatory reactions, affecting allergic rhinitis and chronic rhinosinusitis. CXCL8 is a pro-inflammatory chemokine and a critical factor that causes many airway inflammatory diseases including allergic rhinitis and chronic rhinosinusitis.Materials and Method: Histamine cytotoxicity was measured by MTT assay. Real-time polymerase chain reaction was used to identify histamine type 1 receptor in nasal fibroblasts. The fibroblasts were then treated with histamine with or without a histamine type 1 receptor antagonist and the CXCL8 protein was assessed using an enzyme-linked immunosorbent assay (ELISA). The downstream signaling molecules, including phospholipase C and phospho-p50, were evaluated by western blot and immunofluorescent staining.Results: Histamine had no significant cytotoxic effect until the concentration reached 1,000 μM. Histamine type 1 receptor mRNA was expressed in nasal fibroblasts. CXCL8 protein expression level was significantly increased following histamine stimulation. However, the expression level of CXCL8 decreased when phospholipase C was inhibited by U73122. Histamine increased phospho-p50 expression as seen in western blot results. The BAY11-7082, NF-κB inhibitor significantly reduced CXCL8 production in histamine-stimulated nasal fibroblasts.Conclusion: Histamine can induce the production of NF-κB controlled-chemokine CXCL8 by nasal fibroblasts, which supports a role for histamine in upper airway inflammatory diseases. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | 대한비과학회 | - |
dc.title | Histamine Induced Production of Chemokine CXCL8 Through H1R/PLC and NF-κB Signaling Pathways in Nasal Fibroblasts | - |
dc.title.alternative | Histamine Induced Production of Chemokine CXCL8 Through H1R/PLC and NF-κB Signaling Pathways in Nasal Fibroblasts | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | 이흥만 | - |
dc.identifier.doi | 10.18787/jr.2019.00302 | - |
dc.identifier.bibliographicCitation | Journal of Rhinology, v.27, no.2, pp.95 - 101 | - |
dc.relation.isPartOf | Journal of Rhinology | - |
dc.citation.title | Journal of Rhinology | - |
dc.citation.volume | 27 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 95 | - |
dc.citation.endPage | 101 | - |
dc.type.rims | ART | - |
dc.identifier.kciid | ART002652641 | - |
dc.description.journalClass | 2 | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordAuthor | Histamine | - |
dc.subject.keywordAuthor | CXCL8 | - |
dc.subject.keywordAuthor | Histamine type 1 receptor | - |
dc.subject.keywordAuthor | Signal pathways | - |
dc.subject.keywordAuthor | Nasal fibroblasts. | - |
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