Histamine Induced Production of Chemokine CXCL8 Through H1R/PLC and NF-κB Signaling Pathways in Nasal FibroblastsHistamine Induced Production of Chemokine CXCL8 Through H1R/PLC and NF-κB Signaling Pathways in Nasal Fibroblasts
- Other Titles
- Histamine Induced Production of Chemokine CXCL8 Through H1R/PLC and NF-κB Signaling Pathways in Nasal Fibroblasts
- Authors
- 강병진; 박주후; 이흥만
- Issue Date
- 2020
- Publisher
- 대한비과학회
- Keywords
- Histamine; CXCL8; Histamine type 1 receptor; Signal pathways; Nasal fibroblasts.
- Citation
- Journal of Rhinology, v.27, no.2, pp.95 - 101
- Indexed
- KCI
- Journal Title
- Journal of Rhinology
- Volume
- 27
- Number
- 2
- Start Page
- 95
- End Page
- 101
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/131115
- DOI
- 10.18787/jr.2019.00302
- ISSN
- 1229-1498
- Abstract
- Background and Objectives: Histamine has been suggested to play an important role during allergic and inflammatory reactions, affecting allergic rhinitis and chronic rhinosinusitis. CXCL8 is a pro-inflammatory chemokine and a critical factor that causes many airway inflammatory diseases including allergic rhinitis and chronic rhinosinusitis.Materials and Method: Histamine cytotoxicity was measured by MTT assay. Real-time polymerase chain reaction was used to identify histamine type 1 receptor in nasal fibroblasts. The fibroblasts were then treated with histamine with or without a histamine type 1 receptor antagonist and the CXCL8 protein was assessed using an enzyme-linked immunosorbent assay (ELISA). The downstream signaling molecules, including phospholipase C and phospho-p50, were evaluated by western blot and immunofluorescent staining.Results: Histamine had no significant cytotoxic effect until the concentration reached 1,000 μM. Histamine type 1 receptor mRNA was expressed in nasal fibroblasts. CXCL8 protein expression level was significantly increased following histamine stimulation. However, the expression level of CXCL8 decreased when phospholipase C was inhibited by U73122. Histamine increased phospho-p50 expression as seen in western blot results. The BAY11-7082, NF-κB inhibitor significantly reduced CXCL8 production in histamine-stimulated nasal fibroblasts.Conclusion: Histamine can induce the production of NF-κB controlled-chemokine CXCL8 by nasal fibroblasts, which supports a role for histamine in upper airway inflammatory diseases.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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