Heat Shock Proteins and Autophagy in Rats with Cerulein-Induced Acute Pancreatitis
- Authors
- Kim, Jin Nam; Lee, Hong Sik; Ryu, Soo Hyung; Kim, You Sun; Moon, Jeong Seop; Kim, Chang Duck; Chang, In Youb; Yoon, Sang Pill
- Issue Date
- 12월-2011
- Publisher
- EDITORIAL OFFICE GUT & LIVER
- Keywords
- Acute pancreatitis; Autophagy; Heat shock protein
- Citation
- GUT AND LIVER, v.5, no.4, pp.513 - 520
- Indexed
- SCIE
SCOPUS
KCI
OTHER
- Journal Title
- GUT AND LIVER
- Volume
- 5
- Number
- 4
- Start Page
- 513
- End Page
- 520
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/134207
- DOI
- 10.5009/gnl.2011.5.4.513
- ISSN
- 1976-2283
- Abstract
- Background/Aims: Heat shock proteins (HSPs) protect rats from cerulein-induced acute pancreatitis (AP) by preventing the subcellular redistribution of cathepsin B and the activation of trypsinogen. Autophagy plays a critical role in the secretion of digestive enzymes and triggering of cerulein-induced AP via the colocalization of trypsinogen and lysosomes. Therefore, using a rat cerulein-induced AP model, we investigated whether HSPs prevent AP by regulating autophagy. Methods: Twelve hours after fed standard laboratory chow and water, the experimental groups (cerulein, water-immersion [WI]-cerulein and heat-shock [HS]-cerulein) and the control groups (control, WI, and HS) received one intraperitoneal injection of cerulein (50 mu g/kg) or saline, respectively. All of the rats were sacrificed at 6 hours after injection. The severity of the AP was assessed based on the serum amylase level and the histological and electron microscopy findings. Western blotting was also performed for HSP60/70 and LC3B-II. Results: WI and HS induced HSP60 and HSP70, respectively. The induced HSP60/70 effectively prevented the development of cerulein-induced AP. Autophagy developed in the rats with cerulein-induced AP and was documented by the expression of LC3-II and electron microscopy findings. The WI-stressed rats and HS-treated rats did not develop cerulein-induced autophagy. Conclusions: HSPs exert protective effects against cerulein-induced AP in rats by inhibiting autophagy. (Gut Liver 2011;5:513-520)
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
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