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Single-Cell RNA Sequencing Revealed the Heterogeneity of Gonadal Primordial Germ Cells in Zebra Finch (Taeniopygia guttata)

Authors
Jung, Kyung MinSeo, MinseokKim, Young MinKim, Jin LeeHan, Jae Yong
Issue Date
9-Dec-2021
Publisher
FRONTIERS MEDIA SA
Keywords
zebra finch (Taeniopygia guttata); embryonic gonad; primordial germ cell; single-cell RNA sequencing; heterogeneity
Citation
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, v.9
Indexed
SCIE
SCOPUS
Journal Title
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
Volume
9
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/135446
DOI
10.3389/fcell.2021.791335
ISSN
2296-634X
Abstract
Primordial germ cells (PGCs) are undifferentiated gametes with heterogeneity, an evolutionarily conserved characteristic across various organisms. Although dynamic selection at the level of early germ cell populations is an important biological feature linked to fertility, the heterogeneity of PGCs in avian species has not been characterized. In this study, we sought to evaluate PGC heterogeneity in zebra finch using a single-cell RNA sequencing (scRNA-seq) approach. Using scRNA-seq of embryonic gonadal cells from male and female zebra finches at Hamburger and Hamilton (HH) stage 28, we annotated nine cell types from 20 cell clusters. We found that PGCs previously considered a single population can be separated into three subtypes showing differences in apoptosis, proliferation, and other biological processes. The three PGC subtypes were specifically enriched for genes showing expression patterns related to germness or pluripotency, suggesting functional differences in PGCs according to the three subtypes. Additionally, we discovered a novel biomarker, SMC1B, for gonadal PGCs in zebra finch. The results provide the first evidence of substantial heterogeneity in PGCs previously considered a single population in birds. This discovery expands our understanding of PGCs to avian species, and provides a basis for further research.
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