Linkage between bacterial community-mediated hydrogen peroxide detoxification and the growth of Microcystis aeruginosa
- Authors
- Kim, Minkyung; Kim, Wonjae; Lee, Yunho; Park, Woojun
- Issue Date
- 1-12월-2021
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Catalase; Microcystin; Nanopore sequencing; Oxidative stress; Photosynthesis; RNA-sequencing
- Citation
- WATER RESEARCH, v.207
- Indexed
- SCIE
SCOPUS
- Journal Title
- WATER RESEARCH
- Volume
- 207
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/135504
- DOI
- 10.1016/j.watres.2021.117784
- ISSN
- 0043-1354
- Abstract
- Microcystis aeruginosa, an important cyanobloom-forming cyanobacterium, is sensitive to the high light intensity and consequent oxidative stress. Based on our genomic and transcriptomic analyses of H2O2-treated cells, many genes involved in photosynthesis, Calvin cycle, and microcystin synthesis were downregulated, whereas several toxin-antitoxin genes, DNA repair genes, and H2O2-defense systems such as peroxiredoxins and glutathione synthesis were upregulated. Axenic M. aeruginosa was then co-cultured with synthetic bacterial communities collected from 15 different freshwater samples with exhibiting different degrees of H2O2-production and catalase activities. Our analyses indicated that H2O2-resistant bacterial communities favored the growth and photosynthetic activity of M. aeruginosa cells under either H2O2 treatment or high light conditions. Nanopore-based bacterial community analyses indicated that these growth-promoting effects were likely attributable to a high proportion of Alphaproteobacteria (e.g., Brevundimonas and Ochrobactrum species), which protected M. aeruginosa cells from H2O2 toxicity. Further, these bacterial communities exhibited higher catalase activity levels and faster O-2 production rates upon H(2)O(2 )detoxification. Taken together, our findings newly suggest that the occurrence of catalase-less M. aeruginosa blooms is largely influenced by the surrounding microbiota during high light and organic-rich conditions.
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Collections - College of Life Sciences and Biotechnology > Division of Environmental Science and Ecological Engineering > 1. Journal Articles
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