Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Osteostimulating Ability of beta-tricalcium Phosphate/collagen Composite as a Practical Bone-grafting Substitute: In vitro and in vivo Comparison Study with Commercial One

Authors
Cheon, En-jiKim, Sung-HoLee, Dong-KeonJo, Yun-KeeKi, Mi-RanPark, Chang-JooJang, Hyon-SeokAhn, Jin-SooPack, Seung-PilJun, Sang-Ho
Issue Date
12월-2021
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Keywords
Beta-TCP; bone regeneration; collagen; composite; osteogenesis
Citation
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.26, no.6, pp.923 - 932
Indexed
SCIE
SCOPUS
KCI
Journal Title
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume
26
Number
6
Start Page
923
End Page
932
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/135520
DOI
10.1007/s12257-021-0059-4
ISSN
1226-8372
Abstract
A biocompatible and biodegradable bioceramic, beta-tricalcium phosphate (beta-TCP) has been widely considered as a safe and effective alternative to autograft for bone reconstruction due to its excellent osteoconductivity. Here, we prepared the composite of beta-TCP and collagen (beta-TCP/col) to investigate whether such a bone extracellular matrix (ECM)-mimetic synthetic bone graft can achieve a highly accelerated bone growth comparable to a commercially available bone substitute. The bone regenerating effects of the beta-TCP/col block were evaluated in vitro and in vivo by comparison with Bio-Oss Collagen (R) (Bio-Oss/col). Preosteoblasts showed similar levels of cell adhesion and proliferation in the beta-TCP/col and Bio-Oss/col scaffolds. Expression levels of osteogenic differentiation marker genes, including alkaline phosphatase (ALP), bone sialoprotein (BSP) and osteocalcin (OCN), increased significantly in cells on the collagen plug compared to the control (without any bone graft material). Notably, cells on the beta-TCP/col scaffold exhibited more than 2-fold higher expression levels of ALP and OCN compared to the Bio-Oss/col scaffold. In addition, in vivo animal experiments showed that the beta-TCP/col scaffold induced a remarkably accelerated bone formation (similar to 69.54%) compared to the Bio-Oss/col scaffold (similar to 67.08%) and the collagen plug (similar to 11.47%) at 8 weeks after implantation. These results indicated that the composite of beta-TCP and collagen (beta-TCP/col) showing a comparable bone-regenerating ability could be used as a substitute for the most commercially available Bio-Oss Collagen (R).
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biotechnology and Bioinformatics > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Pack, Seung Pil photo

Pack, Seung Pil
생명정보공학과
Read more

Altmetrics

Total Views & Downloads

BROWSE