Comparative metabolite profiling of wild type and thermo-tolerant mutant of Saccharomyces cerevisiae
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sooah | - |
dc.contributor.author | Shin, Minhye | - |
dc.contributor.author | Choi, Wonja | - |
dc.contributor.author | Kim, Kyoung Heon | - |
dc.date.accessioned | 2022-02-13T01:41:12Z | - |
dc.date.available | 2022-02-13T01:41:12Z | - |
dc.date.created | 2022-02-09 | - |
dc.date.issued | 2021-12 | - |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/135569 | - |
dc.description.abstract | Simultaneous saccharification and fermentation (SSF) is a process of enzymatic saccharification and yeast fermentation used for producing lignocellulosic ethanol, and is performed at high temperature for effective enzymatic saccharification. Thermo-tolerant yeast is needed for high ethanol yield from yeast fermentation. A thermo-tolerant mutant yeast, SSK2, derived from Saccharomyces cerevisiae BY4741, has been generated previously. However, the thermo-tolerance mechanisms in SSK2 remain unclear. In this study, to investigate the change of metabolisms in thermo-tolerant mutant, metabolomics was performed based on gas chromatography/ time-of-flight mass spectrometry (GC/TOF MS). In total, 91 intracellular metabolites were identified by GC/TOF MS; the metabolite profiles of BY4741 and SSK2, cultivated at 30 degrees C and 42 degrees C, were clearly discriminated by principal component analysis (PCA) and hierarchical clustering analysis (HCA). The metabolomics results indicated that the metabolism of amino acids, cell wall and membrane, and fatty acids were changed by heat stress and were key factors in yeast thermo-tolerance. In this study, metabolomics was successfully exploited to reveal the changes of metabolisms in the thermo-tolerant yeast, and these metabolic changes could be used as the clues for future development of thermo-tolerant yeasts. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | ACETIC-ACID | - |
dc.subject | FERMENTATION TEMPERATURE | - |
dc.subject | ETHANOL TOLERANCE | - |
dc.subject | OSMOTIC-STRESS | - |
dc.subject | HEAT-SHOCK | - |
dc.subject | ACCUMULATION | - |
dc.subject | EXPRESSION | - |
dc.subject | TREHALOSE | - |
dc.subject | INCREASE | - |
dc.subject | PROLINE | - |
dc.title | Comparative metabolite profiling of wild type and thermo-tolerant mutant of Saccharomyces cerevisiae | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Kyoung Heon | - |
dc.identifier.doi | 10.1016/j.procbio.2021.10.006 | - |
dc.identifier.scopusid | 2-s2.0-85118704295 | - |
dc.identifier.wosid | 000710727400008 | - |
dc.identifier.bibliographicCitation | PROCESS BIOCHEMISTRY, v.111, pp.62 - 68 | - |
dc.relation.isPartOf | PROCESS BIOCHEMISTRY | - |
dc.citation.title | PROCESS BIOCHEMISTRY | - |
dc.citation.volume | 111 | - |
dc.citation.startPage | 62 | - |
dc.citation.endPage | 68 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.subject.keywordPlus | ACCUMULATION | - |
dc.subject.keywordPlus | ACETIC-ACID | - |
dc.subject.keywordPlus | ETHANOL TOLERANCE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | FERMENTATION TEMPERATURE | - |
dc.subject.keywordPlus | HEAT-SHOCK | - |
dc.subject.keywordPlus | INCREASE | - |
dc.subject.keywordPlus | OSMOTIC-STRESS | - |
dc.subject.keywordPlus | PROLINE | - |
dc.subject.keywordPlus | TREHALOSE | - |
dc.subject.keywordAuthor | Ethanol | - |
dc.subject.keywordAuthor | GC | - |
dc.subject.keywordAuthor | Metabolomics | - |
dc.subject.keywordAuthor | Saccharomycescerevisiae | - |
dc.subject.keywordAuthor | Simultaneous saccharification and | - |
dc.subject.keywordAuthor | TOF-MS | - |
dc.subject.keywordAuthor | Thermotolerance | - |
dc.subject.keywordAuthor | fermentation (SSF) | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.