Increased Production of Colanic Acid by an Engineered Escherichia coli Strain, Mediated by Genetic and Environmental Perturbations
- Authors
- Yun, Eun Ju; Cho, Yoonho; Han, Na Ree; Kim, In Jung; Jin, Yong-Su; Kim, Kyoung Heon
- Issue Date
- 12월-2021
- Publisher
- SPRINGER
- Keywords
- Colanic acid; Environmental perturbation; Escherichia coli; Exopolysaccharide; rcsF; waaF
- Citation
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.193, no.12, pp.4083 - 4096
- Indexed
- SCIE
SCOPUS
- Journal Title
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
- Volume
- 193
- Number
- 12
- Start Page
- 4083
- End Page
- 4096
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/135603
- DOI
- 10.1007/s12010-021-03671-0
- ISSN
- 0273-2289
- Abstract
- Colanic acid (CA) is a major exopolysaccharide synthesized by Escherichia coli that serves as a constituent of biofilm matrices. CA demonstrates potential applications in the food, cosmetics, and pharmaceutical industry. Moreover, L-fucose, a monomeric constituent of CA, exhibits various physiological activities, such as antitumor, anti-inflammatory, and skin-whitening. Here, the effects of genetic and environmental perturbations were investigated for improving CA production by E. coli. When rcsF, a positive regulator gene of CA synthesis, was expressed in E. coli Delta waaF, a CA-producing strain constructed previously, the CA titer increased to 3051.2 mg/L as compared to 2052.8 mg/L observed with E. coli Delta waaF. Among the environmental factors tested, namely, osmotic and oxidative stresses and pH, pH was a primary factor that significantly improved CA production. When the pH of the culture medium of E. coli Delta waaF + rcsF was maintained at 7, the CA titer significantly increased to 4351.6 mg/L. The CA yield obtained with E. coli Delta waaF + rcsF grown at pH 7 was 5180.4 mg CA/g dry cell weight, which is the highest yield of CA reported so far. This engineered E. coli system with optimization of environmental conditions can be employed for fast and economically-feasible production of CA.
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Collections - College of Life Sciences and Biotechnology > Division of Food Bioscience and Technology > 1. Journal Articles
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