Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Performance Evaluation of the BZ COVID-19 Neutralizing Antibody Test for the Culture-Free and Rapid Detection of SARS-CoV-2 Neutralizing Antibodies

Authors
Jung, Bo KyeungYoon, JungBae, Joon-YongKim, JeonghunPark, Man-SeongLee, Suk YongLim, Chae Seung
Issue Date
12월-2021
Publisher
MDPI
Keywords
COVID-19; ELISA; RDT; SARS-CoV-2; neutralizing antibodies
Citation
DIAGNOSTICS, v.11, no.12
Indexed
SCIE
SCOPUS
Journal Title
DIAGNOSTICS
Volume
11
Number
12
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/135648
DOI
10.3390/diagnostics11122193
ISSN
2075-4418
Abstract
Rapid and accurate measurement of SARS-CoV-2 neutralizing antibodies (nAbs) can aid in understanding the development of immunity against COVID-19. This study evaluated the diagnostic performance of a rapid SARS-CoV-2 nAb detection test called the BZ COVID-19 nAb test BZ-nAb (BZ-nAb; BioZentech). Using the 90% plaque-reduction neutralization test (PRNT-90) as a reference, 104 serum specimens collected from COVID-19-positive and -negative patients were grouped into 40 PRNT-90-positive and 64 PRNT-90-negative specimens. The performance of the BZ-nAb was compared with that of the cPass surrogate virus neutralization test (cPass sVNT; Genscript). The BZ-nAb showed a sensitivity ranging from 92.5%-95.0% and specificity ranging from 96.9%-100%, whereas cPass sVNT showed a sensitivity of 100% (95% confidence interval (CI) 90.5%-100%) and specificity of 98.4% (95% CI, 91.6%-100%). The dilution factor obtained with PRNT-90 showed a stronger correlation with the percent inhibition of cPass sVNT (r = 0.8660, p < 0.001) compared with the test and control line ratio (T/C ratio) of the BZ-nAb (r = -0.7089, p < 0.001). An almost perfect agreement was seen between the BZ-nAb and cPass sVNT results, with a strong negative correlation between the BZ-nAb T/C ratio and cPass sVNT percent inhibition (r = -0.8022, p < 0.001). In conclusion, the diagnostic performance of the BZ-nAb was comparable to that of the cPass sVNT, although the BZ-nAb had a slightly lower sensitivity.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biomedical Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Park, Man Seong photo

Park, Man Seong
의과학과
Read more

Altmetrics

Total Views & Downloads

BROWSE