Engineering of Klebsiella oxytoca for the Production of 2,3-Butanediol from High Concentration of Xylose
DC Field | Value | Language |
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dc.contributor.author | Cha, Ji Won | - |
dc.contributor.author | Jang, Seung Hoon | - |
dc.contributor.author | Son, Jaewoo | - |
dc.contributor.author | Kim, Jungyeon | - |
dc.contributor.author | Jung, Inho | - |
dc.contributor.author | Kim, Kyoung Heon | - |
dc.contributor.author | Chang, Yong Keun | - |
dc.contributor.author | Jeong, Ki Jun | - |
dc.date.accessioned | 2022-02-14T23:40:54Z | - |
dc.date.available | 2022-02-14T23:40:54Z | - |
dc.date.created | 2022-02-08 | - |
dc.date.issued | 2021-11-01 | - |
dc.identifier.issn | 2168-0485 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/135797 | - |
dc.description.abstract | Klebsiella oxytoca is widely used for the biological production of 2,3-butanediol (2,3-BDO), a promising platform chemical with a broad range of applications. Here, to improve cell growth and production of 2,3-BDO under high concentration of xylose (100 g/L), we engineered K. oxytoca using an adaptive laboratory evolution and a biosensor-derived high throughput screening strategy. First, we developed a XylR-dependent xylose biosensor for the detection of intracellular xylose, and K. oxytoca containing the xylose biosensor was used for adaptive laboratory evolution in 100 g/L xylose. Cells were isolated by FACS screening, and the isolated strain (KO8S16) showed much improved cell growth with high xylose consumption rate (1.35 g/L/h) and 2,3-BDO productivity (0.53 g/L/h) compared with the wild-type strain. Through whole genome resequencing, it was revealed that a mutation in OmpR (a response regulator of osmotic stress) allowed to withstand high concentrations of xylose. Finally, fed-batch cultivation was performed by feeding high concentration of xylose, and K. oxytoca successfully produced 2,3-BDO at a concentration as high as 57.5 g/L by consuming 238.13 g/L xylose in 47 h. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | GCN5-RELATED N-ACETYLTRANSFERASES | - |
dc.subject | BIOMASS-DERIVED SUGARS | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | ENHANCED PRODUCTION | - |
dc.subject | LIGNOCELLULOSIC BIOMASS | - |
dc.subject | FERMENTATIVE PRODUCTION | - |
dc.subject | BACILLUS-LICHENIFORMIS | - |
dc.subject | PROTEIN | - |
dc.subject | ACETYLATION | - |
dc.subject | HYDROLYSATE | - |
dc.title | Engineering of Klebsiella oxytoca for the Production of 2,3-Butanediol from High Concentration of Xylose | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Kyoung Heon | - |
dc.identifier.doi | 10.1021/acssuschemeng.1c04118 | - |
dc.identifier.scopusid | 2-s2.0-85116283735 | - |
dc.identifier.wosid | 000715216700009 | - |
dc.identifier.bibliographicCitation | ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v.9, no.43, pp.14395 - 14404 | - |
dc.relation.isPartOf | ACS SUSTAINABLE CHEMISTRY & ENGINEERING | - |
dc.citation.title | ACS SUSTAINABLE CHEMISTRY & ENGINEERING | - |
dc.citation.volume | 9 | - |
dc.citation.number | 43 | - |
dc.citation.startPage | 14395 | - |
dc.citation.endPage | 14404 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Green & Sustainable Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.subject.keywordPlus | ACETYLATION | - |
dc.subject.keywordPlus | BACILLUS-LICHENIFORMIS | - |
dc.subject.keywordPlus | BIOMASS-DERIVED SUGARS | - |
dc.subject.keywordPlus | ENHANCED PRODUCTION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | FERMENTATIVE PRODUCTION | - |
dc.subject.keywordPlus | GCN5-RELATED N-ACETYLTRANSFERASES | - |
dc.subject.keywordPlus | HYDROLYSATE | - |
dc.subject.keywordPlus | LIGNOCELLULOSIC BIOMASS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordAuthor | 2,3-butanediol | - |
dc.subject.keywordAuthor | Klebsiella oxytoca | - |
dc.subject.keywordAuthor | adaptive laboratory evolution | - |
dc.subject.keywordAuthor | biosensor | - |
dc.subject.keywordAuthor | xylose | - |
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