Single-step purified R-phycoerythrin transmits cellular imaging functionalities in vitro
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sathuvan, Malairaj | - |
dc.contributor.author | Thangam, Ramar | - |
dc.contributor.author | Venkateshbabu, Gopal | - |
dc.contributor.author | Cheong, Kit-Leong | - |
dc.contributor.author | Kang, Heemin | - |
dc.contributor.author | Liu, Yang | - |
dc.date.accessioned | 2022-02-22T20:42:09Z | - |
dc.date.available | 2022-02-22T20:42:09Z | - |
dc.date.created | 2022-02-11 | - |
dc.date.issued | 2022-01-01 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/136541 | - |
dc.description.abstract | A single-step and rapid chromatographic method-based purification of Gracilaria corticata (J. Agardh) R-phycoerythrin (R-PE) was attained using polyacrylamide gel electrophoresis (PAGE) technique without affecting structural integrity. The purified R-PE had a characteristic UV-Vis spectrum with three absorbance maxima at 496, 535, and 565 nm, and fluorescence at 575 nm. R-PE was obtained with a purity index of 4.2 and a recovery yield of 44.3%. SIDS-PAGE analysis exhibited three sub-units i.e., 18, 21, and 31 kDa, which corresponds to alpha, beta, and gamma, respectively. This report's purification process was considered less time-consuming and could be efficiently applied to purify phycobiliproteins. The purified R-PE showed optimal stability up to 6 h at pH 7.0 when exposed to light (3000 lx), while the temperature at which the maximum stability was retained was at 20 degrees C. The cellular imaging property of R-PE was effectively implemented to evaluate its credentials without affecting the cell proliferation of Vero and Hep-2 cell lines with the higher IC50 concentrations in vitro. Under fluorescence microscopy and flow cytometry analysis, purified R-PE displayed the characteristic affinity towards cell imaging functions in preliminary in vitro studies. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.subject | B-PHYCOERYTHRIN | - |
dc.subject | PALMARIA-PALMATA | - |
dc.subject | PORPHYRIDIUM-CRUENTUM | - |
dc.subject | C-PHYCOERYTHRIN | - |
dc.subject | PURIFICATION | - |
dc.subject | PHYCOCYANIN | - |
dc.subject | PH | - |
dc.subject | PHYCOBILIPROTEINS | - |
dc.subject | SEPARATION | - |
dc.subject | PROTEINS | - |
dc.title | Single-step purified R-phycoerythrin transmits cellular imaging functionalities in vitro | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kang, Heemin | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2021.11.099 | - |
dc.identifier.scopusid | 2-s2.0-85121232561 | - |
dc.identifier.wosid | 000734407300005 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.194, pp.563 - 570 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.citation.volume | 194 | - |
dc.citation.startPage | 563 | - |
dc.citation.endPage | 570 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.subject.keywordPlus | B-PHYCOERYTHRIN | - |
dc.subject.keywordPlus | PALMARIA-PALMATA | - |
dc.subject.keywordPlus | PORPHYRIDIUM-CRUENTUM | - |
dc.subject.keywordPlus | C-PHYCOERYTHRIN | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | PHYCOCYANIN | - |
dc.subject.keywordPlus | PH | - |
dc.subject.keywordPlus | PHYCOBILIPROTEINS | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordAuthor | Gracilaria corticata | - |
dc.subject.keywordAuthor | Phycobiliprotein | - |
dc.subject.keywordAuthor | R-phycoerythrin | - |
dc.subject.keywordAuthor | SDS-PAGE | - |
dc.subject.keywordAuthor | Physico-chemical properties | - |
dc.subject.keywordAuthor | In vitro cell imaging | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.