Single-step purified R-phycoerythrin transmits cellular imaging functionalities in vitro
- Authors
- Sathuvan, Malairaj; Thangam, Ramar; Venkateshbabu, Gopal; Cheong, Kit-Leong; Kang, Heemin; Liu, Yang
- Issue Date
- 1-1월-2022
- Publisher
- ELSEVIER
- Keywords
- Gracilaria corticata; Phycobiliprotein; R-phycoerythrin; SDS-PAGE; Physico-chemical properties; In vitro cell imaging
- Citation
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.194, pp.563 - 570
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
- Volume
- 194
- Start Page
- 563
- End Page
- 570
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/136541
- DOI
- 10.1016/j.ijbiomac.2021.11.099
- ISSN
- 0141-8130
- Abstract
- A single-step and rapid chromatographic method-based purification of Gracilaria corticata (J. Agardh) R-phycoerythrin (R-PE) was attained using polyacrylamide gel electrophoresis (PAGE) technique without affecting structural integrity. The purified R-PE had a characteristic UV-Vis spectrum with three absorbance maxima at 496, 535, and 565 nm, and fluorescence at 575 nm. R-PE was obtained with a purity index of 4.2 and a recovery yield of 44.3%. SIDS-PAGE analysis exhibited three sub-units i.e., 18, 21, and 31 kDa, which corresponds to alpha, beta, and gamma, respectively. This report's purification process was considered less time-consuming and could be efficiently applied to purify phycobiliproteins. The purified R-PE showed optimal stability up to 6 h at pH 7.0 when exposed to light (3000 lx), while the temperature at which the maximum stability was retained was at 20 degrees C. The cellular imaging property of R-PE was effectively implemented to evaluate its credentials without affecting the cell proliferation of Vero and Hep-2 cell lines with the higher IC50 concentrations in vitro. Under fluorescence microscopy and flow cytometry analysis, purified R-PE displayed the characteristic affinity towards cell imaging functions in preliminary in vitro studies.
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