Glucose/Xylose Co-Fermenting Saccharomyces cerevisiae Increases the Production of Acetyl-CoA Derived n-Butanol From Lignocellulosic Biomass
- Authors
- Lee, Yeon-Jung; Hoang Nguyen Tran, Phuong; Ko, Ja Kyong; Gong, Gyeongtaek; Um, Youngsoon; Han, Sung Ok; Lee, Sun-Mi
- Issue Date
- 16-Feb-2022
- Publisher
- FRONTIERS MEDIA SA
- Keywords
- Saccharomyces cerevisiae; glucose; xylose co-fermentation; n-butanol; acetyl-CoA; acetate; lignocellulosic biomass
- Citation
- FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v.10
- Indexed
- SCIE
SCOPUS
- Journal Title
- FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
- Volume
- 10
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/139165
- DOI
- 10.3389/fbioe.2022.826787
- ISSN
- 2296-4185
- Abstract
- Efficient xylose catabolism in engineered Saccharomyces cerevisiae enables more economical lignocellulosic biorefinery with improved production yields per unit of biomass. Yet, the product profile of glucose/xylose co-fermenting S. cerevisiae is mainly limited to bioethanol and a few other chemicals. Here, we introduced an n-butanol-biosynthesis pathway into a glucose/xylose co-fermenting S. cerevisiae strain (XUSEA) to evaluate its potential on the production of acetyl-CoA derived products. Higher n-butanol production of glucose/xylose co-fermenting strain was explained by the transcriptomic landscape, which revealed strongly increased acetyl-CoA and NADPH pools when compared to a glucose fermenting wild-type strain. The acetate supplementation expected to support acetyl-CoA pool further increased n-butanol production, which was also validated during the fermentation of lignocellulosic hydrolysates containing acetate. Our findings imply the feasibility of lignocellulosic biorefinery for producing fuels and chemicals derived from a key intermediate of acetyl-CoA through glucose/xylose co-fermentation.
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