3,4-dihydroxy-L-phenylalanine as a cell adhesion molecule in serum-free cell culture
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 유승호 | - |
dc.date.accessioned | 2022-04-11T14:43:17Z | - |
dc.date.available | 2022-04-11T14:43:17Z | - |
dc.date.created | 2022-04-08 | - |
dc.date.issued | 2012-07 | - |
dc.identifier.issn | 8756-7938 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/140027 | - |
dc.description.abstract | In this article, we examined the feasibility of using 3,4-dihydroxy-L-phenylalanine (DOPA) as a cell adhesion molecule in serum-free cultures of anchorage-dependent mammalian cells. DOPA is a critical, functional element in mussel adhesive proteins and is known to bind strongly to various natural or synthetic materials. DOPA coating on culture plates was confirmed using X-ray photoelectron spectroscopy and energy-dispersive spectroscopy. Human dermal fibroblasts (HDFs) were cultured on DOPA-coated, fibronectin-coated, or no material-coated culture plates in serum-free medium. HDFs cultured on DOPA showed the highest cell adhesion ratio, spreading, and viability but the lowest apoptotic activity. Therefore, DOPA may be a useful cell-adhesion molecule for serum-free culture. © 2012 American Institute of Chemical Engineers (AIChE). | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | 3,4-dihydroxy-L-phenylalanine as a cell adhesion molecule in serum-free cell culture | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | 유승호 | - |
dc.identifier.doi | 10.1002/btpr.1560 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY PROGRESS, v.28, no.4, pp.1055 - 1060 | - |
dc.relation.isPartOf | BIOTECHNOLOGY PROGRESS | - |
dc.citation.title | BIOTECHNOLOGY PROGRESS | - |
dc.citation.volume | 28 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1055 | - |
dc.citation.endPage | 1060 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | 3 | - |
dc.subject.keywordAuthor | 4-dihydroxy-L-phenylalanine | - |
dc.subject.keywordAuthor | cell adhesion | - |
dc.subject.keywordAuthor | cell adhesion molecule | - |
dc.subject.keywordAuthor | dermal fibroblasts | - |
dc.subject.keywordAuthor | serum-free culture | - |
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