3,4-dihydroxy-L-phenylalanine as a cell adhesion molecule in serum-free cell culture
- Authors
- 유승호
- Issue Date
- 7월-2012
- Publisher
- WILEY
- Keywords
- 3; 4-dihydroxy-L-phenylalanine; cell adhesion; cell adhesion molecule; dermal fibroblasts; serum-free culture
- Citation
- BIOTECHNOLOGY PROGRESS, v.28, no.4, pp.1055 - 1060
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOTECHNOLOGY PROGRESS
- Volume
- 28
- Number
- 4
- Start Page
- 1055
- End Page
- 1060
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/140027
- DOI
- 10.1002/btpr.1560
- ISSN
- 8756-7938
- Abstract
- In this article, we examined the feasibility of using 3,4-dihydroxy-L-phenylalanine (DOPA) as a cell adhesion molecule in serum-free cultures of anchorage-dependent mammalian cells. DOPA is a critical, functional element in mussel adhesive proteins and is known to bind strongly to various natural or synthetic materials. DOPA coating on culture plates was confirmed using X-ray photoelectron spectroscopy and energy-dispersive spectroscopy. Human dermal fibroblasts (HDFs) were cultured on DOPA-coated, fibronectin-coated, or no material-coated culture plates in serum-free medium. HDFs cultured on DOPA showed the highest cell adhesion ratio, spreading, and viability but the lowest apoptotic activity. Therefore, DOPA may be a useful cell-adhesion molecule for serum-free culture. © 2012 American Institute of Chemical Engineers (AIChE).
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