Expansion of the prime editing modality with Cas9 from Francisella novicida
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, Yeounsun | - |
dc.contributor.author | Lee, Wi-jae | - |
dc.contributor.author | Hur, Junho K. | - |
dc.contributor.author | Song, Woo Jeung | - |
dc.contributor.author | Lee, Youngjeon | - |
dc.contributor.author | Kim, Hanseop | - |
dc.contributor.author | Gwon, Lee Wha | - |
dc.contributor.author | Kim, Young-Hyun | - |
dc.contributor.author | Park, Young-Ho | - |
dc.contributor.author | Kim, Chan Hyoung | - |
dc.contributor.author | Lim, Kyung-Seob | - |
dc.contributor.author | Song, Bong-Seok | - |
dc.contributor.author | Huh, Jae-Won | - |
dc.contributor.author | Kim, Sun-Uk | - |
dc.contributor.author | Jun, Bong-Hyun | - |
dc.contributor.author | Jung, Cheulhee | - |
dc.contributor.author | Lee, Seung Hwan | - |
dc.date.accessioned | 2022-05-03T04:42:47Z | - |
dc.date.available | 2022-05-03T04:42:47Z | - |
dc.date.created | 2022-05-03 | - |
dc.date.issued | 2022-04-11 | - |
dc.identifier.issn | 1474-760X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/140659 | - |
dc.description.abstract | Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase after nick formation by CRISPR nickase. In this study, we develop a technology that can be used to insert or replace external bases in the target DNA sequence by linking reverse transcriptase to the Francisella novicida Cas9, which is a CRISPR-Cas9 ortholog. Using FnCas9(H969A) nickase, the targeting limitation of existing Streptococcus pyogenes Cas9 nickase [SpCas9(H840A)]-based prime editing is dramatically extended, and accurate prime editing is induced specifically for the target genes in human cell lines. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.subject | GENOMIC DNA | - |
dc.subject | DUAL-RNA | - |
dc.subject | BASE | - |
dc.title | Expansion of the prime editing modality with Cas9 from Francisella novicida | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jung, Cheulhee | - |
dc.identifier.doi | 10.1186/s13059-022-02644-8 | - |
dc.identifier.scopusid | 2-s2.0-85128054199 | - |
dc.identifier.wosid | 000783162000001 | - |
dc.identifier.bibliographicCitation | GENOME BIOLOGY, v.23, no.1 | - |
dc.relation.isPartOf | GENOME BIOLOGY | - |
dc.citation.title | GENOME BIOLOGY | - |
dc.citation.volume | 23 | - |
dc.citation.number | 1 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.subject.keywordPlus | GENOMIC DNA | - |
dc.subject.keywordPlus | DUAL-RNA | - |
dc.subject.keywordPlus | BASE | - |
dc.subject.keywordAuthor | Prime editing | - |
dc.subject.keywordAuthor | Target expansion | - |
dc.subject.keywordAuthor | CRISPR-Cas9 | - |
dc.subject.keywordAuthor | Ortholog | - |
dc.subject.keywordAuthor | Francisella novicida | - |
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