Expansion of the prime editing modality with Cas9 from Francisella novicida
- Authors
- Oh, Yeounsun; Lee, Wi-jae; Hur, Junho K.; Song, Woo Jeung; Lee, Youngjeon; Kim, Hanseop; Gwon, Lee Wha; Kim, Young-Hyun; Park, Young-Ho; Kim, Chan Hyoung; Lim, Kyung-Seob; Song, Bong-Seok; Huh, Jae-Won; Kim, Sun-Uk; Jun, Bong-Hyun; Jung, Cheulhee; Lee, Seung Hwan
- Issue Date
- 11-4월-2022
- Publisher
- BMC
- Keywords
- Prime editing; Target expansion; CRISPR-Cas9; Ortholog; Francisella novicida
- Citation
- GENOME BIOLOGY, v.23, no.1
- Indexed
- SCIE
SCOPUS
- Journal Title
- GENOME BIOLOGY
- Volume
- 23
- Number
- 1
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/140659
- DOI
- 10.1186/s13059-022-02644-8
- ISSN
- 1474-760X
- Abstract
- Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase after nick formation by CRISPR nickase. In this study, we develop a technology that can be used to insert or replace external bases in the target DNA sequence by linking reverse transcriptase to the Francisella novicida Cas9, which is a CRISPR-Cas9 ortholog. Using FnCas9(H969A) nickase, the targeting limitation of existing Streptococcus pyogenes Cas9 nickase [SpCas9(H840A)]-based prime editing is dramatically extended, and accurate prime editing is induced specifically for the target genes in human cell lines.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
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