Accelerated sunlight-driven conversion of industrial flue gas into biofuels by microfluidic high-throughput screening towards improving photosynthesis in microalgae under fluctuating light
DC Field | Value | Language |
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dc.contributor.author | Sung, Young Joon | - |
dc.contributor.author | Lee, Jeong Seop | - |
dc.contributor.author | Sim, Sang Jun | - |
dc.date.accessioned | 2022-06-09T06:40:30Z | - |
dc.date.available | 2022-06-09T06:40:30Z | - |
dc.date.created | 2022-06-09 | - |
dc.date.issued | 2022-09-01 | - |
dc.identifier.issn | 1385-8947 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/141701 | - |
dc.description.abstract | Sunlight-driven microalgal conversion of CO2 is a promising carbon neutral strategy for the sustainable production of various value-added products. However, its real-world application is challenging due to the irregularity of sunlight which reduces photosynthetic efficiency. Here, we report that microdroplet-based screening of photosynthesis-augmented microalgae mutant under fluctuating light accelerates mass production of CO2- derived algal biofuel in natural sunlight. Random insertional Chlamydomonas reinhardtii mutants were cultivated in single-layered microdroplet photobioreactors to be effectively applied to light conditions mimicking natural sunlight without self-shading. High-density microdroplets containing fast-growing mutants were efficiently selected at the single-droplet level by a centrifugation-assisted droplet sorting platform for high-throughput screening. Consequently, we isolated a mutant exhibiting cell growth 1.85-fold that of the wild-type even under continuous and rapid alteration in light intensity, which can serve as a cell stressor. After whole-genome resequencing, we found disruption in the SNF2 gene encoding ATP-dependent chromatin remodeling complexes, which reveal its importance for the regulation of chlorophyll biosynthesis and accumulation of reactive oxygen species under fluctuating light. After a 1.6 ton-scale field culture utilizing natural sunlight and industrial CO2, the mutant showed increased biomass productivity, CO2 fixation rate, and calorific value, a crucial parameter of fuel performance, by 34.38%, 45.07%, and 16.82%, respectively. Our results indicate that improving photosynthesis of microalgae in fluctuating light environments elucidates the mechanisms responsible for enhancing the sunlight utilization, allowing industrial-scale biological CO2 conversion. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.title | Accelerated sunlight-driven conversion of industrial flue gas into biofuels by microfluidic high-throughput screening towards improving photosynthesis in microalgae under fluctuating light | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sim, Sang Jun | - |
dc.identifier.doi | 10.1016/j.cej.2022.136487 | - |
dc.identifier.scopusid | 2-s2.0-85129549437 | - |
dc.identifier.wosid | 000799989400005 | - |
dc.identifier.bibliographicCitation | CHEMICAL ENGINEERING JOURNAL, v.443 | - |
dc.relation.isPartOf | CHEMICAL ENGINEERING JOURNAL | - |
dc.citation.title | CHEMICAL ENGINEERING JOURNAL | - |
dc.citation.volume | 443 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Engineering, Environmental | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.subject.keywordPlus | CHLAMYDOMONAS-REINHARDTII | - |
dc.subject.keywordPlus | LIPID PRODUCTION | - |
dc.subject.keywordPlus | WASTE-WATER | - |
dc.subject.keywordPlus | BIODIESEL | - |
dc.subject.keywordPlus | PHOTOBIOREACTOR | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BIOMASS | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | OXYGEN | - |
dc.subject.keywordAuthor | Biological conversion of CO2 | - |
dc.subject.keywordAuthor | Microalgae | - |
dc.subject.keywordAuthor | Gel microdroplet-based screening | - |
dc.subject.keywordAuthor | SNF2 gene | - |
dc.subject.keywordAuthor | Biofuel | - |
dc.subject.keywordAuthor | Flue gas | - |
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