Improving the robustness of a catalyzed hairpin assembly with a three-arm nanostructure for nonenzymatic signal amplification
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Eunjoo | - |
dc.contributor.author | Xu, Jiaxin | - |
dc.contributor.author | Kim, Jinah | - |
dc.contributor.author | Chun, Honggu | - |
dc.date.accessioned | 2022-06-09T14:40:33Z | - |
dc.date.available | 2022-06-09T14:40:33Z | - |
dc.date.created | 2022-06-09 | - |
dc.date.issued | 2022-05-03 | - |
dc.identifier.issn | 0003-2654 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/141741 | - |
dc.description.abstract | Kinetically trapped hairpin DNA has great potential to dynamically build nanostructures, which can be initiated by sequence-specific nucleic acids. The branched junction, which has a multi-arm structure, is a representative nanostructure of DNA. In this study, we report a nonenzymatic and isothermal signal amplification accompanied by building a 3-arm structure based on a catalyzed hairpin DNA assembly (3-CHA). We improved the signal-to-background ratio of the 3-CHA by suppressing the leakage pathway of 3-CHA, thus eliminating unfavorable reaction sites exposed in the single-stranded region of hairpin DNAs. Background and amplified signals were analyzed with gel electrophoresis and real-time fluorescence monitoring. The limit of detection of the developed 3-CHA was estimated to be 29.3 pM for catalyst DNA at room temperature. Supported by the reduced leakage signal, the implemented 3-CHA showed great potential for detecting low concentrations of target DNA. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | HYBRIDIZATION CHAIN-REACTION | - |
dc.subject | FREE FLUORESCENCE APTASENSOR | - |
dc.subject | ENZYME-FREE | - |
dc.subject | NUCLEIC-ACIDS | - |
dc.subject | LOGIC GATE | - |
dc.subject | LABEL-FREE | - |
dc.subject | DNA | - |
dc.subject | MICRORNA | - |
dc.subject | CIRCUITS | - |
dc.subject | JUNCTIONS | - |
dc.title | Improving the robustness of a catalyzed hairpin assembly with a three-arm nanostructure for nonenzymatic signal amplification | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chun, Honggu | - |
dc.identifier.doi | 10.1039/d2an00209d | - |
dc.identifier.scopusid | 2-s2.0-85128357694 | - |
dc.identifier.wosid | 000774029800001 | - |
dc.identifier.bibliographicCitation | ANALYST, v.147, no.9, pp.1899 - 1905 | - |
dc.relation.isPartOf | ANALYST | - |
dc.citation.title | ANALYST | - |
dc.citation.volume | 147 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1899 | - |
dc.citation.endPage | 1905 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | HYBRIDIZATION CHAIN-REACTION | - |
dc.subject.keywordPlus | FREE FLUORESCENCE APTASENSOR | - |
dc.subject.keywordPlus | ENZYME-FREE | - |
dc.subject.keywordPlus | NUCLEIC-ACIDS | - |
dc.subject.keywordPlus | LOGIC GATE | - |
dc.subject.keywordPlus | LABEL-FREE | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | MICRORNA | - |
dc.subject.keywordPlus | CIRCUITS | - |
dc.subject.keywordPlus | JUNCTIONS | - |
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