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Improving the robustness of a catalyzed hairpin assembly with a three-arm nanostructure for nonenzymatic signal amplification

Authors
Kim, EunjooXu, JiaxinKim, JinahChun, Honggu
Issue Date
3-5월-2022
Publisher
ROYAL SOC CHEMISTRY
Citation
ANALYST, v.147, no.9, pp.1899 - 1905
Indexed
SCIE
SCOPUS
Journal Title
ANALYST
Volume
147
Number
9
Start Page
1899
End Page
1905
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/141741
DOI
10.1039/d2an00209d
ISSN
0003-2654
Abstract
Kinetically trapped hairpin DNA has great potential to dynamically build nanostructures, which can be initiated by sequence-specific nucleic acids. The branched junction, which has a multi-arm structure, is a representative nanostructure of DNA. In this study, we report a nonenzymatic and isothermal signal amplification accompanied by building a 3-arm structure based on a catalyzed hairpin DNA assembly (3-CHA). We improved the signal-to-background ratio of the 3-CHA by suppressing the leakage pathway of 3-CHA, thus eliminating unfavorable reaction sites exposed in the single-stranded region of hairpin DNAs. Background and amplified signals were analyzed with gel electrophoresis and real-time fluorescence monitoring. The limit of detection of the developed 3-CHA was estimated to be 29.3 pM for catalyst DNA at room temperature. Supported by the reduced leakage signal, the implemented 3-CHA showed great potential for detecting low concentrations of target DNA.
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