Production of Polyhydroxyalkanoates with the Fermentation of Methylorubrum extorquens Using Formate as a Carbon Substrate
DC Field | Value | Language |
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dc.contributor.author | Chang, Woojin | - |
dc.contributor.author | Yoon, Jihee | - |
dc.contributor.author | Oh, Min-Kyu | - |
dc.date.accessioned | 2022-06-10T17:40:29Z | - |
dc.date.available | 2022-06-10T17:40:29Z | - |
dc.date.created | 2022-06-09 | - |
dc.date.issued | 2022-04 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/141876 | - |
dc.description.abstract | Deriving both carbon and energy from formate, a single-carbon substrate, for the microbial production of value-added products allows its use as the main feedstock in biorefinery, with consequent environmental and economic benefits. Methylorubrum extorquens AM1, a strain capable of growth solely on formate, is a known producer of the biopolymer polyhydroxyalkanoate (PHA), during nitrogen-deficient growth. Based on findings from our previous report, the gene ftfL was selected for overexpression to enhance growth and PHA production using formate. Its overexpression in a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)-producing strain yielded improved growth and a 1.4-fold increase in PHBV production. The strain could thus be tested for long-term fermentation, intended for optimized growth and production in formate. A customized fermentation regimen was established by incorporating both the conventional two-phase fermentation method for PHA production and a repeated fed-batch fermentation process designed to resolve the problem of sodium accumulation. The poly-3-hydroxybutyrate and PHBV copolymer-producing strains resulted in 11.07 g/L and 2.76 g/L of bio-degradable polymers, respectively, in the fermentation process. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.subject | PHB PRODUCTION | - |
dc.subject | METHANOL | - |
dc.subject | ASSIMILATION | - |
dc.subject | CO2 | - |
dc.subject | AM1 | - |
dc.title | Production of Polyhydroxyalkanoates with the Fermentation of Methylorubrum extorquens Using Formate as a Carbon Substrate | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Min-Kyu | - |
dc.identifier.doi | 10.1007/s12257-021-0218-7 | - |
dc.identifier.scopusid | 2-s2.0-85128708156 | - |
dc.identifier.wosid | 000787180700004 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.27, no.2, pp.268 - 275 | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 27 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 268 | - |
dc.citation.endPage | 275 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002837408 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | PHB PRODUCTION | - |
dc.subject.keywordPlus | METHANOL | - |
dc.subject.keywordPlus | ASSIMILATION | - |
dc.subject.keywordPlus | CO2 | - |
dc.subject.keywordPlus | AM1 | - |
dc.subject.keywordAuthor | Methylorubrum extorquens | - |
dc.subject.keywordAuthor | formate | - |
dc.subject.keywordAuthor | polyhydroxyalkanoate | - |
dc.subject.keywordAuthor | repeated fed-batch fermentation | - |
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