Production of Polyhydroxyalkanoates with the Fermentation of Methylorubrum extorquens Using Formate as a Carbon Substrate
- Authors
- Chang, Woojin; Yoon, Jihee; Oh, Min-Kyu
- Issue Date
- 4월-2022
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- Keywords
- Methylorubrum extorquens; formate; polyhydroxyalkanoate; repeated fed-batch fermentation
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.27, no.2, pp.268 - 275
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- Volume
- 27
- Number
- 2
- Start Page
- 268
- End Page
- 275
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/141876
- DOI
- 10.1007/s12257-021-0218-7
- ISSN
- 1226-8372
- Abstract
- Deriving both carbon and energy from formate, a single-carbon substrate, for the microbial production of value-added products allows its use as the main feedstock in biorefinery, with consequent environmental and economic benefits. Methylorubrum extorquens AM1, a strain capable of growth solely on formate, is a known producer of the biopolymer polyhydroxyalkanoate (PHA), during nitrogen-deficient growth. Based on findings from our previous report, the gene ftfL was selected for overexpression to enhance growth and PHA production using formate. Its overexpression in a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)-producing strain yielded improved growth and a 1.4-fold increase in PHBV production. The strain could thus be tested for long-term fermentation, intended for optimized growth and production in formate. A customized fermentation regimen was established by incorporating both the conventional two-phase fermentation method for PHA production and a repeated fed-batch fermentation process designed to resolve the problem of sodium accumulation. The poly-3-hydroxybutyrate and PHBV copolymer-producing strains resulted in 11.07 g/L and 2.76 g/L of bio-degradable polymers, respectively, in the fermentation process.
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