Production of human spinal-cord organoids recapitulating neural-tube morphogenesis
- Authors
- Lee, Ju-Hyun; Shin, Hyogeun; Shaker, Mohammed R.; Kim, Hyun Jung; Park, Si-Hyung; Kim, June Hoan; Lee, Namwon; Kang, Minjin; Cho, Subin; Kwak, Tae Hwan; Kim, Jong Woon; Song, Mi-Ryoung; Kwon, Seung-Hae; Han, Dong Wook; Lee, Sanghyuk; Choi, Se-Young; Rhyu, Im Joo; Kim, Hyun; Geum, Dongho; Cho, Il-Joo; Sun, Woong
- Issue Date
- Apr-2022
- Publisher
- NATURE PORTFOLIO
- Citation
- NATURE BIOMEDICAL ENGINEERING, v.6, no.4, pp.435 - +
- Indexed
- SCIE
SCOPUS
- Journal Title
- NATURE BIOMEDICAL ENGINEERING
- Volume
- 6
- Number
- 4
- Start Page
- 435
- End Page
- +
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/141878
- DOI
- 10.1038/s41551-022-00868-4
- ISSN
- 2157-846X
- Abstract
- Human spinal-cord-like tissues induced from human pluripotent stem cells are typically insufficiently mature and do not mimic the morphological features of neurulation. Here, we report a three-dimensional culture system and protocol for the production of human spinal-cord-like organoids (hSCOs) recapitulating the neurulation-like tube-forming morphogenesis of the early spinal cord. The hSCOs exhibited neurulation-like tube-forming morphogenesis, cellular differentiation into the major types of spinal-cord neurons as well as glial cells, and mature synaptic functional activities, among other features of the development of the spinal cord. We used the hSCOs to screen for antiepileptic drugs that can cause neural-tube defects. hSCOs may also facilitate the study of the development of the human spinal cord and the modelling of diseases associated with neural-tube defects. A protocol for the production of human spinal-cord-like organoids that recapitulate the tube-forming morphogenesis of the early human spinal cord facilitates screening for antiepileptic drugs that can cause neural-tube defects.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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