Multiplex SNP Genotyping Using SWITCH: Sequence-Specific Nanoparticle with Interpretative Toehold-Mediated Sequence Decoding in Hydrogel
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Woongsun | - |
dc.contributor.author | Park, Eunhye | - |
dc.contributor.author | Bae, Seojin | - |
dc.contributor.author | Choi, Kyung-Hak | - |
dc.contributor.author | Han, Sangeun | - |
dc.contributor.author | Son, Kuk-Hui | - |
dc.contributor.author | Lee, Do Young | - |
dc.contributor.author | Cho, Il-Joo | - |
dc.contributor.author | Seong, Hyejeong | - |
dc.contributor.author | Hwang, Kyo Seon | - |
dc.contributor.author | Nam, Jwa-Min | - |
dc.contributor.author | Choi, Jungkyu | - |
dc.contributor.author | Lee, Hyojin | - |
dc.contributor.author | Choi, Nakwon | - |
dc.date.accessioned | 2022-06-12T10:41:03Z | - |
dc.date.available | 2022-06-12T10:41:03Z | - |
dc.date.created | 2022-06-10 | - |
dc.date.issued | 2022-02 | - |
dc.identifier.issn | 1613-6810 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/142128 | - |
dc.description.abstract | Single nucleotide polymorphisms (SNPs) that can alter phenotypes of individuals play a pivotal role in disease development and, more importantly, responses to therapy. However, SNP genotyping has been challenging due to the similarity of SNP alleles and their low concentration in biological samples. Sequence-specific nanoparticle with interpretative toehold-mediated sequence decoding in hydrogel (SWITCH) for multiplex SNP genotyping is presented. The encoding with gold nanoparticle probes transduces each SNP target to approximate to 1000 invaders with prominently different sequences between wild and mutant types, featuring polymerase chain reaction (PCR)-free amplification. Subsequently, the toehold-mediated DNA replacement in hydrogel microparticles decodes the invaders via SNP-specific fluorescence signals. The 4-plex detection of the warfarin-associated SNP targets spiked in commercially validated human serum (S1-100ML, Merck) is successfully demonstrated with excellent specificity. This work is the first technology development presenting PCR-free, multiplex SNP genotyping with a single reporting fluorophore, to the best of knowledge. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Multiplex SNP Genotyping Using SWITCH: Sequence-Specific Nanoparticle with Interpretative Toehold-Mediated Sequence Decoding in Hydrogel | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Jungkyu | - |
dc.contributor.affiliatedAuthor | Choi, Nakwon | - |
dc.identifier.doi | 10.1002/smll.202105538 | - |
dc.identifier.scopusid | 2-s2.0-85121448497 | - |
dc.identifier.wosid | 000731409800001 | - |
dc.identifier.bibliographicCitation | SMALL, v.18, no.8 | - |
dc.relation.isPartOf | SMALL | - |
dc.citation.title | SMALL | - |
dc.citation.volume | 18 | - |
dc.citation.number | 8 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.relation.journalWebOfScienceCategory | Physics, Condensed Matter | - |
dc.subject.keywordPlus | ALLELE-SPECIFIC PCR | - |
dc.subject.keywordPlus | DNA PURIFICATION | - |
dc.subject.keywordPlus | STRAND-DISPLACEMENT | - |
dc.subject.keywordPlus | POLYMORPHISMS | - |
dc.subject.keywordPlus | DISCOVERY | - |
dc.subject.keywordPlus | VKORC1 | - |
dc.subject.keywordPlus | MICROPARTICLES | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | ASSOCIATION | - |
dc.subject.keywordPlus | GENETICS | - |
dc.subject.keywordAuthor | gold nanoparticles | - |
dc.subject.keywordAuthor | hydrogel microparticles | - |
dc.subject.keywordAuthor | magnetic microparticles | - |
dc.subject.keywordAuthor | multiplex single nucleotide polymorphism genotyping | - |
dc.subject.keywordAuthor | sandwich assays | - |
dc.subject.keywordAuthor | toehold-mediated DNA displacement | - |
dc.subject.keywordAuthor | warfarin | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.