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Multiplex SNP Genotyping Using SWITCH: Sequence-Specific Nanoparticle with Interpretative Toehold-Mediated Sequence Decoding in Hydrogelopen access

Authors
Choi, WoongsunPark, EunhyeBae, SeojinChoi, Kyung-HakHan, SangeunSon, Kuk-HuiLee, Do YoungCho, Il-JooSeong, HyejeongHwang, Kyo SeonNam, Jwa-MinChoi, JungkyuLee, HyojinChoi, Nakwon
Issue Date
2월-2022
Publisher
WILEY-V C H VERLAG GMBH
Keywords
gold nanoparticles; hydrogel microparticles; magnetic microparticles; multiplex single nucleotide polymorphism genotyping; sandwich assays; toehold-mediated DNA displacement; warfarin
Citation
SMALL, v.18, no.8
Indexed
SCIE
SCOPUS
Journal Title
SMALL
Volume
18
Number
8
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/142128
DOI
10.1002/smll.202105538
ISSN
1613-6810
Abstract
Single nucleotide polymorphisms (SNPs) that can alter phenotypes of individuals play a pivotal role in disease development and, more importantly, responses to therapy. However, SNP genotyping has been challenging due to the similarity of SNP alleles and their low concentration in biological samples. Sequence-specific nanoparticle with interpretative toehold-mediated sequence decoding in hydrogel (SWITCH) for multiplex SNP genotyping is presented. The encoding with gold nanoparticle probes transduces each SNP target to approximate to 1000 invaders with prominently different sequences between wild and mutant types, featuring polymerase chain reaction (PCR)-free amplification. Subsequently, the toehold-mediated DNA replacement in hydrogel microparticles decodes the invaders via SNP-specific fluorescence signals. The 4-plex detection of the warfarin-associated SNP targets spiked in commercially validated human serum (S1-100ML, Merck) is successfully demonstrated with excellent specificity. This work is the first technology development presenting PCR-free, multiplex SNP genotyping with a single reporting fluorophore, to the best of knowledge.
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Choi, Jung kyu
공과대학 (화공생명공학과)
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