Development of a fluorescent nanoprobe based on an amphiphilic single-benzene-based fluorophore for lipid droplet detection and its practical applications
- Authors
- Jung, Yuna; Jin, Ji Hye; Kim, Youngseo; Oh, Ji Hyeon; Moon, Heechang; Jeong, Huisu; Kim, Jaehoon; Park, Yoon Kyung; Oh, Yohan; Park, Sungnam; Kim, Dokyoung
- Issue Date
- 13-7월-2022
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- ORGANIC & BIOMOLECULAR CHEMISTRY, v.20, no.27, pp.5423 - 5433
- Indexed
- SCIE
SCOPUS
- Journal Title
- ORGANIC & BIOMOLECULAR CHEMISTRY
- Volume
- 20
- Number
- 27
- Start Page
- 5423
- End Page
- 5433
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/142755
- DOI
- 10.1039/d2ob00830k
- ISSN
- 1477-0520
- Abstract
- Lipid droplets (LDs) are crucial biological organelles connected with metabolic pathways in biological systems and diseases. To monitor the locations and accumulation of LDs in lipid-related diseases, the development of a visualization tool for LDs has gained importance. In particular, LD visualization using fluorescent probes has gained attention. Herein, a new fluorescent nanoprobe, BMeS-Ali, is developed that can sense LDs based on an amphiphilic single benzene-based fluorophore (SBBF). BMeS-Ali consists of hydrophilic (-NH2) and hydrophobic (-C12H25) moieties and exists as a micelle nanostructure in aqueous media. BMeS-Ali has a weak fluorescence, but its emission was dramatically enhanced upon exposure to the LD components such as oleic acids (OA) by reassembling its nano-formulation. BMeS-Ali showed a selective LD staining ability and great biocompatibility in cells (cancer cells and stem cells). It also showed a practical sensing ability towards biologically derived lipids and can be applied to the visualization of human fingerprints. We found that the nanoprobe BMeS-Ali has significant potential to serve as a practical dye and sensor for lipids, especially for LD imaging in the biomedical research area and broader industrial applications.
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