Protein Hydrolysate from Spirulina platensis Prevents Dexamethasone-Induced Muscle Atrophy via Akt/Foxo3 Signaling in C2C12 Myotubes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Chi-Woo | - |
dc.contributor.author | Chang, Yeok Boo | - |
dc.contributor.author | Park, Chun Woong | - |
dc.contributor.author | Han, Sung Hee | - |
dc.contributor.author | Suh, Hyung Joo | - |
dc.contributor.author | Ahn, Yejin | - |
dc.date.accessioned | 2022-08-13T10:40:37Z | - |
dc.date.available | 2022-08-13T10:40:37Z | - |
dc.date.created | 2022-08-12 | - |
dc.date.issued | 2022-06 | - |
dc.identifier.issn | 1660-3397 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/143020 | - |
dc.description.abstract | Loss of muscle mass is the primary symptom of sarcopenia. Protein intake is recommended to prevent muscle mass loss, and Spirulina platensis, a microalga with high protein content, is a potential protein supplement. Here, we evaluated the differentiation ability of C2C12 cells and the inhibitory effect of Spirulina hydrolysates (SPH) prepared by Collupulin on dexamethasone (DEX)-treated C2C12 cells. SPH contained 578.27 mg/g protein and 92.30 mg/g branched-chain amino acids. SPH increased C2C12 myotube length and diameter, likely owing to increased MyoD1 and Myf5 expression. Inhibition of increased Atrogin-1, MuRF-1, and FoxO3 expression by SPH in DEX-treated C2C12 cells suppressed DEX-induced muscle atrophy. Moreover, SPH inhibited the DEX-induced increase in cytosolic p-Akt protein expression and suppressed the increase in nuclear FoxO3a protein expression, thereby suppressing the increase in the protein expression of the ubiquitin-proteasome-related factors Atrogin-1 and MuRF-1, which are involved in muscle atrophy. SPH suppressed DEX-induced muscle atrophy by activating the Akt/FoxO3a pathway. SPH promoted C2C12 myoblast differentiation into myotubes and inhibited DEX-induced myotube atrophy by suppressing Atrogin-1 and MuRF-1 expression and regulating the FoxO3a transcription factor. Collectively, SPH can be used as a functional food to inhibit muscle atrophy and promote muscle regeneration. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | CHAIN AMINO-ACIDS | - |
dc.subject | TRANSCRIPTION FACTORS | - |
dc.subject | ATROGIN-1 | - |
dc.subject | SARCOPENIA | - |
dc.subject | NUTRITION | - |
dc.subject | EXERCISE | - |
dc.subject | PATHWAY | - |
dc.subject | MURF1 | - |
dc.title | Protein Hydrolysate from Spirulina platensis Prevents Dexamethasone-Induced Muscle Atrophy via Akt/Foxo3 Signaling in C2C12 Myotubes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Suh, Hyung Joo | - |
dc.identifier.doi | 10.3390/md20060365 | - |
dc.identifier.scopusid | 2-s2.0-85131175730 | - |
dc.identifier.wosid | 000817503900001 | - |
dc.identifier.bibliographicCitation | MARINE DRUGS, v.20, no.6 | - |
dc.relation.isPartOf | MARINE DRUGS | - |
dc.citation.title | MARINE DRUGS | - |
dc.citation.volume | 20 | - |
dc.citation.number | 6 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | CHAIN AMINO-ACIDS | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTORS | - |
dc.subject.keywordPlus | ATROGIN-1 | - |
dc.subject.keywordPlus | SARCOPENIA | - |
dc.subject.keywordPlus | NUTRITION | - |
dc.subject.keywordPlus | EXERCISE | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | MURF1 | - |
dc.subject.keywordAuthor | Spirulina | - |
dc.subject.keywordAuthor | Collupulin | - |
dc.subject.keywordAuthor | muscle atrophy | - |
dc.subject.keywordAuthor | dexamethasone | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.