Augmentation of the RNA m6A reader signature is associated with poor survival by enhancing cell proliferation and EMT across cancer types

Abstract

Cancer: The role of modifications to RNA Studying the effects of a chemical modification of messenger RNA molecules (mRNA), which carry genetic information from DNA to the cell's protein-making machinery, reveals new insights into the role of these modifications in cancer, suggesting potential therapeutic approaches. Researchers in Seoul, South Korea, led by Joon-Yong An at Korea University and Sung-Yup Cho at Seoul National University investigated the most common modifications of mRNA involving methyl groups (CH3): addition ('writing'), having a regulatory effect on the cell ('reading') or removal ('erasing'). The molecular activities involved in reading the modifications were increased in all 11 types of cancer in cancer-sampling databases and their own patient cohort. Changes in writing and erasing of the modifications varied with cancer type. The proteins that mediate the reading responses to RNA methylation are possible targets for new anti-cancer drugs. N-6-Methyladenosine (m6A) RNA modification plays a critical role in the posttranscriptional regulation of gene expression. Alterations in cellular m6A levels and m6A-related genes have been reported in many cancers, but whether they play oncogenic or tumor-suppressive roles is inconsistent across cancer types. We investigated common features of alterations in m6A modification and m6A-related genes during carcinogenesis by analyzing transcriptome data of 11 solid tumors from The Cancer Genome Atlas database and our in-house gastric cancer cohort. We calculated m6A writer (W), eraser (E), and reader (R) signatures based on corresponding gene expression. Alterations in the W and E signatures varied according to the cancer type, with a strong positive correlation between the W and E signatures in all types. When the patients were divided according to m6A levels estimated by the ratio of the W and E signatures, the prognostic effect of m6A was inconsistent according to the cancer type. The R and especially the R2 signatures (based on the expression of IGF2BPs) were upregulated in all cancers. Patients with a high R2 signature exhibited poor prognosis across types, which was attributed to enrichment of cell cycle- and epithelial-mesenchymal transition-related pathways. Our study demonstrates common features of m6A alterations across cancer types and suggests that targeting m6A R proteins is a promising strategy for cancer treatment.