Through-skull brain imaging in vivo at visible wavelengths via dimensionality reduction adaptive-optical microscopyopen access
- Authors
- Jo, Yonghyeon; Lee, Ye-Ryoung; Hong, Jin Hee; Kim, Dong-Young; Kwon, Junhwan; Choi, Myunghwan; Kim, Moonseok; Choi, Wonshik
- Issue Date
- 29-7월-2022
- Publisher
- AMER ASSOC ADVANCEMENT SCIENCE
- Citation
- SCIENCE ADVANCES, v.8, no.30
- Indexed
- SCIE
SCOPUS
- Journal Title
- SCIENCE ADVANCES
- Volume
- 8
- Number
- 30
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/143840
- DOI
- 10.1126/sciadv.abo4366
- ISSN
- 2375-2548
- Abstract
- Compensation of sample-induced optical aberrations is crucial for visualizing microscopic structures deep within biological tissues. However, strong multiple scattering poses a fundamental limitation for identifying and correcting the tissue-induced aberrations. Here, we introduce a label-free deep-tissue imaging technique termed dimensionality reduction adaptive-optical microscopy (DReAM) to selectively attenuate multiple scattering. We established a theoretical framework in which dimensionality reduction of a time-gated reflection matrix can attenuate uncorrelated multiple scattering while retaining a single-scattering signal with a strong wave correlation, irrespective of sample-induced aberrations. We performed mouse brain imaging in vivo through the intact skull with the probe beam at visible wavelengths. Despite the strong scattering and aberrations, DReAM offered a 17-fold enhancement of single scattering-to-multiple scattering ratio and provided high-contrast images of neural fibers in the brain cortex with the diffraction-limited spatial resolution of 412 nanometers and a 33-fold enhanced Strehl ratio.
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Collections - College of Science > Department of Physics > 1. Journal Articles
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