Anti-obesity effects of isoacteoside on 3T3-L1 adipocytesopen access
- Authors
- Choi, Chang Geun; Lee, Deok Jae; Chung, Namhyun; Joo, Yong Hoon
- Issue Date
- 12월-2022
- Publisher
- SPRINGER SINGAPORE PTE LTD
- Keywords
- Anti-obesity; Isoacteoside; Oil Red O assay; Phenylethanoid glycosides
- Citation
- APPLIED BIOLOGICAL CHEMISTRY, v.65, no.1
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- APPLIED BIOLOGICAL CHEMISTRY
- Volume
- 65
- Number
- 1
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/146480
- DOI
- 10.1186/s13765-022-00701-1
- ISSN
- 2468-0834
- Abstract
- Isoacteoside is a caffeoyl phenylethanoide glycoside found in various plant parts, such as the flower of Magnolia denudata. In particular, magnolia has been studied for its anti-obesity, anticancer, and anti-inflammatory effects. However, isoacteoside has not been extensively studied, except for its anti-inflammatory effects. In this study, the anti-obesity effects of isoacteoside were investigated in 3T3-L1 mouse pre-adipocytes. Isoacteoside treatment did not induce cytotoxicity in 3T3-L1 cells up to a concentration of 1000 mu M. The anti-obesity effects on 3T3-L1 cells were confirmed using oil red O staining. In addition, the expression of obesity-related proteins and genes, such as peroxisome proliferator-activated acceptor gamma (PPAR gamma), CCAAT/enhancer-binding protein alpha (C/EBP alpha), and perilipin (PLIN1), was determined by western blotting and qRT-PCR assays to confirm the anti-obesity effects of isoacteoside. The results of this study suggest that isoacteoside, a natural substance isolated from plant extracts, is not highly toxic to normal cells, such as pre-adipocytes, and displays anti-obesity effects in vitro.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
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