Efficient Synthesis of Stearidonic Acid Enriched Triacylglycerol from Ahiflower Seed Oil via a Two-Step Enzyme Reaction
DC Field | Value | Language |
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dc.contributor.author | Ju, C. | - |
dc.contributor.author | Lee, Y.J. | - |
dc.contributor.author | Yoon, H.S. | - |
dc.contributor.author | Kim, B.H. | - |
dc.contributor.author | Kim, I.-H. | - |
dc.date.accessioned | 2022-12-11T08:41:05Z | - |
dc.date.available | 2022-12-11T08:41:05Z | - |
dc.date.created | 2022-12-08 | - |
dc.date.issued | 2022 | - |
dc.identifier.issn | 1345-8957 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/146965 | - |
dc.description.abstract | Stearidonic acid (SDA) is a plant-based n-3 polyunsaturated fatty acid with multiple biological activities. The enrichment of SDA and synthesis of triacylglycerol (TAG) were carried out consecutively via two lipase-catalyzed reactions, hydrolysis, and esterification. First, SDA was enriched into a glyceride fraction from ahiflower seed oil by Candida rugosa lipase-catalyzed hydrolysis. Under the optimum conditions of 35℃, 0.1% lipase powder of Lipase OF, and 50% buffer solution (based on the weight of total substrate), SDA was enriched from 21.6 to 40.7 wt% in glyceride fraction. SDA-enriched TAG was then synthesized from the SDA-enriched glyceride and the SDA-enriched fatty acid via esterification using an in-house immobilized lipase as a biocatalyst. The SDA-enriched fatty acid was obtained from part of the SDA-enriched glyceride by saponification and the in-house immobilized lipase was prepared from Eversa® Transform 2.0 using Lewatit VP OC 1600 as a carrier. The optimum reaction conditions for the synthesis of TAG were a temperature of 50℃, an enzyme loading of 10%, and a vacuum of 10 mmHg. A maximum conversion to TAG of ca. 94% was achieved after 12 h under the optimum conditions. © 2022 by Japan Oil Chemists’ Society. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | Japan Oil Chemists Society | - |
dc.title | Efficient Synthesis of Stearidonic Acid Enriched Triacylglycerol from Ahiflower Seed Oil via a Two-Step Enzyme Reaction | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, I.-H. | - |
dc.identifier.doi | 10.5650/jos.ess22215 | - |
dc.identifier.scopusid | 2-s2.0-85140609759 | - |
dc.identifier.bibliographicCitation | Journal of Oleo Science, v.71, no.11, pp.1679 - 1688 | - |
dc.relation.isPartOf | Journal of Oleo Science | - |
dc.citation.title | Journal of Oleo Science | - |
dc.citation.volume | 71 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1679 | - |
dc.citation.endPage | 1688 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | ahiflower seed oil | - |
dc.subject.keywordAuthor | Candida rugosa | - |
dc.subject.keywordAuthor | Eversa® transform 2.0 | - |
dc.subject.keywordAuthor | stearidonic acid | - |
dc.subject.keywordAuthor | triacylglycerol | - |
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