Maintenance and differentiation of human ES cells on polyvinylidene fluoride scaffolds immobilized with a vitronectin-derived peptide
- Authors
- Park, Sang Eun; Yeon, Gyu-Bum; Goo, Hui-Gwan; Seo, Dong Sik; Dayem, Ahmed A.; Lee, Kyung Eun; Park, Hyun-Mee; Cho, Ssang-Goo; Kim, Dae-Sung
- Issue Date
- May-2021
- Publisher
- WILEY
- Keywords
- cardiac differentiation; human embryonic stem cells; long-term culture; polyvinylidene fluoride; scaffolds
- Citation
- JOURNAL OF CELLULAR PHYSIOLOGY, v.236, no.5, pp.3510 - 3520
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CELLULAR PHYSIOLOGY
- Volume
- 236
- Number
- 5
- Start Page
- 3510
- End Page
- 3520
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/49412
- DOI
- 10.1002/jcp.30095
- ISSN
- 0021-9541
- Abstract
- Polyvinylidene fluoride (PVDF) is biocompatible, easy to fabricate, and has piezoelectric properties; it has been used for many biomedical applications including stem cell engineering. However, long-term cultivation of human embryonic stem cells (hESCs) and their differentiation toward cardiac lineages on PVDF have not been investigated. Herein, PVDF nanoscaled membrane scaffolds were fabricated by electrospinning; a vitronectin-derived peptide-mussel adhesive protein fusion (VNm) was immobilized on the scaffolds. hESCs cultured on the VNm-coated PVDF scaffold (VNm-PVDF scaffold) were stably expanded for more than 10 passages while maintaining the expression of pluripotency markers and genomic integrity. Under cardiac differentiation conditions, hESCs on the VNm-PVDF scaffold generated more spontaneously beating colonies and showed the upregulation of cardiac-related genes, compared with those cultured on Matrigel and VNm alone. Thus, VNm-PVDF scaffolds may be suitable for the long-term culture of hESCs and their differentiation into cardiac cells, thus expanding their application in regenerative medicine.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
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