Selection and characterization of toxic Aspergillus spore-specific DNA aptamer using spore-SELEX
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seo, Jin-Woo | - |
dc.contributor.author | Kim, Jee Young | - |
dc.contributor.author | Kim, Da Hee | - |
dc.contributor.author | Oh, Jeong-Joo | - |
dc.contributor.author | Kim, Young Jun | - |
dc.contributor.author | Kim, Gyu-Hyeok | - |
dc.date.accessioned | 2021-08-30T04:00:21Z | - |
dc.date.available | 2021-08-30T04:00:21Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2021-01-22 | - |
dc.identifier.issn | 2046-2069 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/50072 | - |
dc.description.abstract | As airborne spores of toxic Aspergillus species cause mild symptoms to invasive fungal infections, their indoor concentration should be controlled through real-time management. Aptamer-based biosensors could provide economical and simple solutions for point-of-care. In this study, we isolated aptamers binding to the spores of three representative toxic Aspergillus species (A. fumigatus, A. flavus, and A. niger) for the first time, using cell-SELEX (systematic evolution of ligands through exponential enrichment). Among the aptamer candidates, Asp-3 showed a broad and high binding affinity for the Aspergillus spores. Considering the low binding affinity with proteinase-treated spores, we speculated that the Asp-3 binding sites could be possibly associated with cell surface proteins. The high Asp-3 specificity was confirmed by comparing the binding affinity between the Aspergillus target species and other common indoor fungal species. Moreover, we also established quantitative linear relationships between Asp-3 and the spore concentration of each Aspergillus species. Therefore, the selected Asp-3 aptamer, conjugated with detection sensors, could be an effective biorecognition element for the spores of three toxic Aspergillus species. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | FUNGAL-INFECTIONS | - |
dc.subject | INDOOR | - |
dc.subject | IDENTIFICATION | - |
dc.subject | ASSOCIATIONS | - |
dc.subject | BACTERIAL | - |
dc.subject | DAMPNESS | - |
dc.title | Selection and characterization of toxic Aspergillus spore-specific DNA aptamer using spore-SELEX | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Gyu-Hyeok | - |
dc.identifier.doi | 10.1039/d0ra09571k | - |
dc.identifier.scopusid | 2-s2.0-85099746185 | - |
dc.identifier.wosid | 000609865700005 | - |
dc.identifier.bibliographicCitation | RSC ADVANCES, v.11, no.5, pp.2608 - 2615 | - |
dc.relation.isPartOf | RSC ADVANCES | - |
dc.citation.title | RSC ADVANCES | - |
dc.citation.volume | 11 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 2608 | - |
dc.citation.endPage | 2615 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | FUNGAL-INFECTIONS | - |
dc.subject.keywordPlus | INDOOR | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | ASSOCIATIONS | - |
dc.subject.keywordPlus | BACTERIAL | - |
dc.subject.keywordPlus | DAMPNESS | - |
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