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Selection and characterization of toxic Aspergillus spore-specific DNA aptamer using spore-SELEX

Authors
Seo, Jin-WooKim, Jee YoungKim, Da HeeOh, Jeong-JooKim, Young JunKim, Gyu-Hyeok
Issue Date
22-1월-2021
Publisher
ROYAL SOC CHEMISTRY
Citation
RSC ADVANCES, v.11, no.5, pp.2608 - 2615
Indexed
SCIE
SCOPUS
Journal Title
RSC ADVANCES
Volume
11
Number
5
Start Page
2608
End Page
2615
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/50072
DOI
10.1039/d0ra09571k
ISSN
2046-2069
Abstract
As airborne spores of toxic Aspergillus species cause mild symptoms to invasive fungal infections, their indoor concentration should be controlled through real-time management. Aptamer-based biosensors could provide economical and simple solutions for point-of-care. In this study, we isolated aptamers binding to the spores of three representative toxic Aspergillus species (A. fumigatus, A. flavus, and A. niger) for the first time, using cell-SELEX (systematic evolution of ligands through exponential enrichment). Among the aptamer candidates, Asp-3 showed a broad and high binding affinity for the Aspergillus spores. Considering the low binding affinity with proteinase-treated spores, we speculated that the Asp-3 binding sites could be possibly associated with cell surface proteins. The high Asp-3 specificity was confirmed by comparing the binding affinity between the Aspergillus target species and other common indoor fungal species. Moreover, we also established quantitative linear relationships between Asp-3 and the spore concentration of each Aspergillus species. Therefore, the selected Asp-3 aptamer, conjugated with detection sensors, could be an effective biorecognition element for the spores of three toxic Aspergillus species.
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KIM, Gyu Hyeok
생명과학대학 (환경생태공학부)
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