Decoding the Capability of Lactobacillus plantarum W1 Isolated from Soybean Whey in Producing an Exopolysaccharide

Abstract

This study aims at producing exopolysaccharides (EPS) from a lactic acid bacterial strain. The soybean whey-isolated Lactobacillus plantarum W1 (EPS-W1), which belongs to genus Lactobacillus, is identified using the phenylalanyl-tRNA sequencing method. Of all the examined strains, R-49778 (as numbered by BCCM/LMG Bacteria Collection, Ghent University, Belgium) showed the highest capability of producing exopoly-saccharides. Structural characterization revealed a novel exopolysaccharide consisting of repeating units of -> 6)-D-Glcp-(1 ->; -> 3)-D-Manp-(1 ->; -> 3)-6-Glcp-(1 -> and a branch of -> 6)-D-Manp-(1 ->; -> 2)-D-Glcp-(1 ->. This discovery opens up avenues for the production of EPS for food industries, functional foods, and biomedical applications.