Codonopsis lanceolata Contributes to Ca (2+) Homeostasis by Mediating SOCE and PLC/IP (3) Pathways in Vascular Endothelial and Smooth Muscle Cells
- Authors
- Kim, Min Kyung; Han, A. Young; Shin, You Kyoung; Lee, Kwang-Won; Seol, Geun Hee
- Issue Date
- 12월-2020
- Publisher
- GEORG THIEME VERLAG KG
- Keywords
- Codonopsis lanceolate; Campanulaceae; store-operated Ca; (2+); entry; inositol 1; 4; 5-trisphosphate; vascular endothelial cells; vascular smooth muscle cells
- Citation
- PLANTA MEDICA, v.86, no.18, pp.1345 - 1352
- Indexed
- SCIE
SCOPUS
- Journal Title
- PLANTA MEDICA
- Volume
- 86
- Number
- 18
- Start Page
- 1345
- End Page
- 1352
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/51372
- DOI
- 10.1055/a-1214-6718
- ISSN
- 0032-0943
- Abstract
- Codonopsis lanceolata has been widely used as an anti-inflammatory and anti-lipogenic agent in traditional medicine. Recently, C. lanceolata was reported to prevent hypertension by improving vascular function. This study evaluated the effects of C. lanceolata and its major component lancemaside A on cytosolic calcium concentration in vascular endothelial cells and vascular smooth muscle cells. Cytosolic calcium concentration was measured using fura-2 AM fluorescence. C. lanceolata or lancemaside A increased the cytosolic calcium concentration by releasing Ca (2+) from the endoplasmic reticulum and sarcoplasmic reticulum and by Ca (2+) entry into endothelial cells and vascular smooth muscle cells from extracellular sources. The C. lanceolata - and lancemaside A-induced cytosolic calcium concentration increases were significantly inhibited by lanthanum, an inhibitor of non-selective cation channels, in both endothelial cells and vascular smooth muscle cells. Moreover, C. lanceolata and lancemaside A significantly inhibited store-operated Ca (2+) entry under pathological extracellular Ca (2+) levels. In Ca (2+) -free extracellular fluid, increases in the cytosolic calcium concentration induced by C. lanceolata or lancemaside A were significantly inhibited by U73122, an inhibitor of phospholipase C, and 2-APB, an inositol 1,4,5-trisphosphate receptor antagonist. In addition, dantrolene treatment, which inhibits Ca (2+) release through ryanodine receptor channels, also inhibited C. lanceolata - or lancemaside A-induced increases in the cytosolic calcium concentration through the phospholipase C/inositol 1,4,5-trisphosphate pathway. These results suggest that C. lanceolata and lancemaside A increase the cytosolic calcium concentration through the non-selective cation channels and phospholipase C/inositol 1,4,5-trisphosphate pathways under physiological conditions and inhibit store-operated Ca (2+) entry under pathological conditions in endothelial cells and vascular smooth muscle cells. C. lanceolata or lancemaside A can protect endothelial cells and vascular smooth muscle cells by maintaining cytosolic calcium concentration homeostasis, suggesting possible applications for these materials in diets for preventing vascular damage.
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